Sequences from confirmation amplicon


Confirmation sequences for line 246D12 and hit At1g30475 are shown.
Line ID 246D12
Locus At1g30475 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10789284
Primers used for PCR GE71 - 8474, show primer details
   
Sequence Name 66-K100941-0022-246-D12C-8409
GenBank Accession LN511556 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K100941-0022-246-D12C-8409
ATTTACATTGAACAGTTGTCGTCTCTAGAATAAAAGACCAGTCTTTGCATGTAACTCTGC
GCTGGTAGGTTCCATTCATGACTTTGCAGCCTTTAAGTTTAAGTGTTTTAAATCTTTAGC
TGTTGGGATAACTTCATCACACACCATTTTGCTCACATAACTTAATTACACATCATAAGC
CATTTTGCTTGTTTTATGAACAAGATCCTTTTGCTCTTATGGCCGATAATAGTTTATTTT
CCGGTATTCTCTTCCTAAGTGTCTCTGAAACGCTAGCTGTTTACTTATTCTGATTTGTTT
TCTGCAGAATTCAAAATGCAGTCAAGGACAAACACAGACCGTTACACGGCAATCACCAAC
AATCACACAAGCTCCTACTCATGGTAAATTGTTTCATCCCCTTCTCTATGTTGAACAACT
ATNTTGCGTTTCTAACAAACAAGAGGGGCTTAAAAAACCATACGATTCTNGCTAATATGA
AACAGTTATTTTGAACTCAAATTGTGGCTTGCTATG
   
Sequence Name 66-K100949-0022-246-D12C-GE71
GenBank Accession LN511557 [GenBank]
Sequencing primer gene specific primer, GE71: 5'-AGAATAAGAACCATAGCAAGCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K100949-0022-246-D12C-GE71
CTGTTTCTATAGCTAGAATCGTATGGTTTTTTAGCCCCTCTTGTTTGTTAGAAACGCAAA
ATAGTTGTTCAACATAGAGAAGGGGATGAAACAATTTACCATGAGTAGGAGCTTGTGTGA
TTGTTGGTGATTGCCGTGTAACGGTCTGTGTTTGTCCTTGACTGCATTTTGAATTCTGCA
GAAAACAAATCAGAATAAGTAAACAGCTAGCGTTTCAGAGACACTTAGGAAGAGAATACC
GGAAAATAAACTATTATCGGCCATAAGAGCAAAAGGATCTTGTTCATAAAACAAGCAAAA
TGGCTTATGATGTGTAATTAAGTTATGTGAGCAAAATGGTGTGTGATGAAGTTATCCCAA
CAGCTAAAGATTTAAAACACTTAAACTTAAAGGCTGCAAAGTCATGAATGGAACCTACCA
GCGCAGAGTTACATGCAAAGACTGGTCTTTTATTCTTAGAGACGACAACTGGTTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11