Sequences from confirmation amplicon


Confirmation sequences for line 247C03 and hit At1g70140 are shown.
Line ID 247C03
Locus At1g70140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26415070
Primers used for PCR 08di - 8474, show primer details
   
Sequence Name 32-K115349-0022-247-C03C-08di
GenBank Accession LN500705 [GenBank]
Sequencing primer gene specific primer, 08di: 5'-CAAGACCATTCTCATCAAGAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115349-0022-247-C03C-08di
AACTCGTGATCCTTCTCTGCCAAGTAGTCGTAGTCACCGCCGCCGACGTCATCGGAGGAG
GAGGATACGGAGGTAAAGTGTTTTCGACTCTGACTGGTCCAACTCTGTCTCTCCGTCTCC
GTGCGATGATACATCTTTGGAGGCAGAAGAAGAAAACTCCAGCTACAAGTAAAGTACTTG
CTGCTGTTATAAGGACAGCTTTGGTGATTGTTTTTTTATCCGAAGATGATGAATTATTCG
ACGGCGGAGATGGGTTTGACGAAGGTGGAAGAAGCGGTGGTGGAACAGGGGAGAGTGAAG
AGATGGGGAAGAAAGTTTCGATATTTTGAGGAGAATCAAATTGAGAAAGTGATTGGAATG
GTAAAACGACGATGAAGAAGAAGTAAATTAGGGTTAAATTAGGCCATGGATGATTAAACT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 16-K115348-0022-247-C03C-8409
GenBank Accession LN493557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115348-0022-247-C03C-8409
CATTTCAGTTTATCATCCATGGCCTAATTTAACCCTAATTTACTTCTTCTTCATCGTCGT
TTTACCATTCCAATCACTTTCTCAATTTGATTCTCCTCAAAATATCGAAACTTTCTTCCC
CATCTCTTCACTCTCCCCTGTTCCACCACCGCTTCTTCCACCTTCGTCAAACCCATCTCC
GCCGTCGAATAATTCATCATCTTCGGATAAAAAAACAATCACCAAAGCTGTCCTTATAAC
AGCAGCAAGTACTTTACTTGTAGCTGGAGTTTTCTTCTTCTGCCTCCAAAGATGTATCAT
CGCACGGAGACGGAGAGACAGAGTTGGACCAGTCAGAGTCGAAAACACTTTACCTCCGTA
TCCTCCTCCTCCGATGACGTCGGCGGCGGTGACTACGACTACTTTGGCTAGAGAAGGATT
CACGAGGTTTGGTGGTGTGAAAGGTTTGATTCTTGATGAAATGGGGTCTTG
Primers used for PCR 83wr - 8474, show primer details
   
Sequence Name 23-K118578-0022-247-C03C-83wr
GenBank Accession LN496763 [GenBank]
Sequencing primer gene specific primer, 83wr: 5'-AAACCATGCCTTTTTGTTTTACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118578-0022-247-C03C-83wr
ATAATTTAATTTGAACTTAATTTTCTCTAGGAATGGTGATGATCCACTACCACTATCATT
GATTTCATTCCATATTCCTTTGACCGACTGAAATTACGTTGGAAATAGTATATTTTGATG
AATAATTTATTTACTCGGAAAAAAGAGGTCAAGTTATTAATAGTAAGTACATATACATTA
TCAATTAAGAATTCAATTGAGTTTTAAGGAAAATCCTATTAATTTGTTTGGTATTCGGTA
TTTGTTAGTTCTAAGGAATTGAATTTCCCGATTATACATCATTATAACGTTCTCAAGTTC
CAAACTTGCAACCCACATTTTGTCGATATTCTCAAATGTGAATTCATTCAATTTCCCATA
GAAAACATAAATTTGCACTTAAAGTTAACAATTGAAATCGTATCTAAATGGGAATGTTTT
TGGCTTTTAGTGTTAGACTTCCAAAGCGTCAAAAATATTTCTAGAAAGAGCACAAAAAAT
AAGCAACGCCACTACTTTTGGACAAAGTCAACGATAACACACATCAACCGCACCAGCTCC
ATAAAAGTCCATCTCACGAAAACGATTCTAGTCAAACTACCTAAAACACCCTTATATTTA
CATACAACCCAATCCCACTAACAAGGGTATTTTCGTCAATCACAAAATTTATCACCGACC
CGGGAAGAAGAAGAAGAACAGATCAACTAATTTCTGCTTTCAACTCCACATTAAACCAAA
ACCTCCAAAAAGAATCATTTATTTAAATTATCTTCCCGTTTTAAGTTCCTGAGATTTTTG
GGATTTGTAAATTTGAAAGAAATTAAACAAAGACGTGGTTTCATTTTTT
   
Sequence Name 07-K118577-0022-247-C03C-8409
GenBank Accession LN489482 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118577-0022-247-C03C-8409
GAGATAGAGAGAGATCAATAAAGGAAACAAAAAAAAAAATGAAAACCCGCTCTTTGTTTA
ATTTTCTTCAAATTTACAATTCCCAAAAATCTCAGGAACTTAAAACGGGAAAATAATTTA
AATAAATGATTCTTTTTGGAGGTTTTGGTTTAATGGGGAGTTAAAAGCAAAAATTAGTTG
ATCTGTTCTTCTTCTTCTCCCCGGGTCGGTGATAAATTTTGTGATTGACAAAAATCCCCT
TGTTAGGGGAATGGGGTTGTATGTAAATATAAGGGTGTTTTAGGTAGTTTGACTAAAATC
GTTTTCGTGAAAGGGACTTTTATGGACCTGGGGCGGTTAATGTGTGTTATCGTTAACTTT
GCCCAAAAGTAGGGGCGTTGCTTATTTTTTGGGCCCTTTCAAAAAATATTTTTGACCCTT
TGAAAGTCTAACACTAAAAGCCAAAAACTTTCCCTTTTAAATACAATTTCAATTGTTAAC
TTTAAGTGCAAATTTATGTTTTCTATGGAAAATTGAATGAATTCACTTTTAAAAATATCA
ACAAAATGTGGGTTGCAAGTTTGAAACTTGAAAACGTTATAATGATGTATAATCGGGAAA
TTCAATTCCTTAAAACTAACAAATCCCGAATACCAAACAAATTAATAGGATTTCCCTAAA
AACTCAATTGAATTCTTAATTGATAATGTATATGTCCTTACAATAAATAACTTGACCCCT
TTTTCCCAAGTAAATAAATTATCCATCAAAATAT

Last Updated on 06.12.2018 16:11