Sequences from confirmation amplicon


Confirmation sequences for line 247G08 and hit At5g28237 are shown.
Line ID 247G08
Locus At5g28237 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10212236
Primers used for PCR PP38 - 8474, show primer details
   
Sequence Name 42-K103008-0022-247-G08C-8409
GenBank Accession LN504426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103008-0022-247-G08C-8409
CCATTTACATTGAATGAAAACCCAACAGTTTTAGTATATACTAGATTTTAACCCGCGGTA
TACCACGGGTCTATATTTTATTTTAGTGTTGACAGTAATTACTTTCGTAATATATAATTC
ACAGTCTTATAATTATTTGTTATTTTTTATATTTTGTATATTTAGATATATTATTTAGTA
TGTTTATAGAATTGAAATTTGATTATTTTTTTGTATCTAGATATTAACCCGCGGTATACC
GCGGGCTTATATTTTCATTAATTAAAAAATAATTGAATATTTTGTAGGATGATTTAATAA
ATTTATGTTATTGTTTGATTTAAATGTATAATTCACCACGAGACGAAGTTTTATAACTCA
AATTTTAAAGTTTTAAGTTGTATTTGTTTATTTTGTTAAATGTTTAATATTGTATAATTG
TATTTTGATTGTTGTTTCTCGGATTTCACCCGTAGTACACCATCCCATATNAATATCGAA
TCAGACCCGTCAATTCTAGGATTTCACCCGTGTGAGTATTTAAGGG
   
Sequence Name 90-K103009-0022-247-G08C-PP38
GenBank Accession LN515035 [GenBank]
Sequencing primer gene specific primer, PP38: 5'-TAAATACTACCACGGGTGAAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K103009-0022-247-G08C-PP38
TGGGATGGTGTATACGGGTGAAATCCGAGAAAAACAATCAAAATACAATTATACAATATT
AAACATTTAACAAAATAAACAAATACAACTTAAAACTTTAAAATTTGAGTTATAAAATTT
CGTCTCGTGGTGAATTATACATTTAAATCAAACAATAACATAAATTTATTAAATCATCCT
ACAAAATATTCAATTATTTTTTAATTAATGAAAATATAAGCCCGCGGTATACCGCGGGTT
AATATCTAGATACAAAAAAATAATCAAATTTCAATTCTATAAACATACTAAATAATATAT
CTNAATATACAAAATATAAAAAATAACAAATAATTATAAGACTGTGAATTATATATTACG
AAAGTAATTACTGTCAACACTAAAATAAAATATAGACCCGTGGTATACCGCGGGTTAAAA
TCTAGTATATACTAAAACTGTTGGGTTTTCATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTNTTATAAAATGAAAGTAC
ATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTNTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11