Sequences from confirmation amplicon


Confirmation sequences for line 248C07 and hit At1g10400 are shown.
Line ID 248C07
Locus At1g10400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3414894
Primers used for PCR 13gp - 8474, show primer details
   
Sequence Name 24-K111730-0022-248-C07C-13gp
GenBank Accession LN497052 [GenBank]
Sequencing primer gene specific primer, 13gp: 5'-ACAGGTTTTATTTTGGAATTGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111730-0022-248-C07C-13gp
AATTTTAAATATATAATATGCTGTTTAATAGGGGTTAAAACTTCAGTTTTTTAACAATTG
TCTCATGGATTTATGGTATAGCGTTACTTTATAACAATTTTAAATTGTAAAATATAAATA
TTAATAATAAAATTTGCAAATTTTAATATATATTACTTTAAAAAATAAATCGTCCCGCGA
TATACCGCGGGTTAAAATCTAGTATTAATATATAGGTGTTAACATACATCATCATGTTCC
ATTGTTACAAAACTCGTTGGTTCGTTCATCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K111729-0022-248-C07C-8409
GenBank Accession LN489791 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111729-0022-248-C07C-8409
ATTTCATTGAATAATCCTGATGAACGAACCACGAGTTTTGTAACAATGGAACATGATGAT
GTATGTTAACACCTATATATTAATACTAGATTTTAACCCGCGGTATATCGCGGGACGATT
TATTTTTTAAAGTAATATATATTAAAATTTGCAAATTTTATTATTAATATTTATATTTTA
CAATTTAAAATTGTTATAAAGTAACGCTATACCATAAATCCATGAGACAATTGTTAAAAA
ACTGAAGTTTTAACCCCTATTAAAACAGCATATTAATAATATTTTAAAATTTTATAAATA
TTTATTCAAATACATCTACCATATAACCCAATTCCAAAATAAAACCTG

Last Updated on 06.12.2018 16:11