Sequences from confirmation amplicon


Confirmation sequences for line 248F06 and hit At2g04030 are shown.
Line ID 248F06
Locus At2g04030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1284508
Primers used for PCR 14gp - 8474, show primer details
   
Sequence Name 25-K111730-0022-248-F06C-14gp
GenBank Accession LN497508 [GenBank]
Sequencing primer gene specific primer, 14gp: 5'-GAGAGCTTGTGAGTCTTTTATGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111730-0022-248-F06C-14gp
GTCATTTTATCTGACTATGTTACCTAATCTGTTTGCAGTTCCCGAGATATCTGAGCTTTG
TGAAGGGAGTTGTGGACTCTGATGATCTTCCTCTTAATGTTTCTCGTGAAATTCTCCAAG
AAAGCAGAATTGTGAGTGGTGCAACTGTTAGTGATACAAATGTTTATCATATATGAATAT
CACAAATTGTAACTGTACTATCTTTCGTGATAATGTTCAGGTAAGAATCATGAGAAAGAG
GCTCATTAGAAAAACCTTTGACATGATACAAGAAATCTCTGAGAGTGAAAACAAAGAGGT
AGGTATCTCATTATCCTAATTTTGTTTTGAGCTCATTTGAAAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CG
   
Sequence Name 09-K111729-0022-248-F06C-8409
GenBank Accession LN490242 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111729-0022-248-F06C-8409
TGAGATACCTACCTCTTTGTTTTCACTCTCAGAGATTTCTTGTATCATGTCAAAGGTTTT
TCTAATGAGCCTCTTTCTCATGATTCTTACCTGAACATTATCACGAAAGATAGTACAGTT
ACAATTTGTGATATTCATATATGATAAACATTTGTATCACTAACAGTTGCACCACTCACA
ATTCTGCTTTCTTGGAGAATTTCACGAGAAACATTAAGAGGAAGATCATCAGAGTCCACA
ACTCCCTTCACAAAGCTCAGATATCTCGGGAACTGCAAACAGATTAGGTAACATAGTCAG
ATAAAAATTGAACCACAAATCTAAAGAGATTTCTACATAAGAAAGAACCCATAAAAGACT
CACAAGCTCT

Last Updated on 06.12.2018 16:11