Sequences from confirmation amplicon


Confirmation sequences for line 249B11 and hit At3g09735 are shown.
Line ID 249B11
Locus At3g09735 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2987224
Primers used for PCR PP37 - 8474, show primer details
   
Sequence Name 43-K103008-0022-249-B11C-8409
GenBank Accession LN504799 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K103008-0022-249-B11C-8409
GCCTTTACATTGAATATATCCTGCAGATTACTGCAGATTAACTAACACTGACAAGCACGA
ATATCATGAAACGAATACCACAAGTTATAATATCCATAGATTGTACAATTTGGGAATGTA
TAAACCATCATATTCGTTATTCACCATGAACCTTAAACTCTGAAACAGCCAAAAATGAAA
AAACTTGACATTCCATGGCAAGATCAAGATGAAAGTGATGAAGTTAACCAAAGTCCTCAA
ACTCAAAATGTATGAAATTCTAAAGAAAAAACAAAACTTTACCGATCCAATTGTGCTAGG
TCCTAACCCATGCTAAATTTCAGAGAATTTGATCATCCATCGTTAAGAAGCGTTACAACA
ATCATACACGTATTGACCAAAATGCTCGAACATAAACAAAAAAAGCTCGCAATCGCAAAT
CTTAAACCGGAACATCATGCATCAAAACCAGAAGATCAGACTCAAGCAACAC
   
Sequence Name 91-K103009-0022-249-B11C-PP37
GenBank Accession LN515114 [GenBank]
Sequencing primer gene specific primer, PP37: 5'-TGTGTTGCTTGAGTCTGATCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K103009-0022-249-B11C-PP37
CGATTGCGAGCTTTTTTTGTTTATGTTCGAGCATTTTGGTCAATACGTGTATGATTGTTG
TAACGCTTCTTAACGATGGATGATCAAATTCTCTGAAATTTAGCATGGGTTAGGACCTAG
CACAATTGGATCGGTAAAGTTTTGTTTTTTCTTTAGAATTTCATACATTTTGAGTTTGAG
GACTTTGGTTAACTTCATCACTTTCATCTTGATCTTGCCATGGAATGTCAAGTTTTTTCA
TTTTTGGCTGTTTCAGAGTTTAAGGTTCATGGTGAATAACGAATATGATGGTTTATACAT
TCCCAAATTGTACAATCTATGGATATTATAACTTGTGGTATTCGTTTCATGATATTCGTG
CTTGTCAGTGTTAGTTAATCTGCAGTAATCTGCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGT

Last Updated on 06.12.2018 16:11