Sequences from confirmation amplicon


Confirmation sequences for line 250B03 and hit At5g05060 are shown.
Line ID 250B03
Locus At5g05060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1494792
Primers used for PCR PP35 - 8474, show primer details
   
Sequence Name 05-K103785-0022-250-B03C-PP35
GenBank Accession LN488322 [GenBank]
Sequencing primer gene specific primer, PP35: 5'-AAACGTGATGTAGAGTGTGATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K103785-0022-250-B03C-PP35
CCTTGACAGTTCCGTTTTATCCCCTAGCACAATTATTTCGTCGAAAATCAGCAAAAAACC
AACATCGACTAGATTAGTCATTAGAGATTACTAACCGTCTTGTCGTTGTAGAAGGAGATA
GCCATGTTGGACAACCTGTTGAGCAAATCTCTGTTGGTCTCATGAGGCTCGCTAACCATA
TTGTCGTCGTCGAGATGAACAGCTCCGCGACCAAATCTGTGACGCACTTTCGAGAAGTCC
ACATCAAAACCCTGAAAACACCACAAATCAAAATTAAAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGAT
   
Sequence Name 21-K103786-0022-250-B03C-8409
GenBank Accession LN495629 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K103786-0022-250-B03C-8409
ATTTACAATTGAATATATCCTTTAATTTTGATTTGTGGTGTTTTCAGGGTTTTGATGTGG
ACTTCTCGAAAGTGCGTCACAGATTTGGTCGCGGAGCTGTTCATCTCGACGACGACNATA
TGGTTAGCGAGCCTCATGAGACCAACAGAGATTTGCTCAACAGGTTGTCCAACATGGCTA
TCTCCTTCTACAACGACAAGACGGTTAGTAATCTCTAATGACTAATCTAGTCGATGTTGG
TTTTTTGCTGATTTTCGACGAAATAATTGTGCTAGGGGATAAAACTGGAACTTGTCAAGG
TTTTGAGAGCAAATTTTCACCCATCTGCTGCAATCACACTCTACATCACGTT
Primers used for PCR PP36 - 8474, show primer details
   
Sequence Name 37-K104195-0022-250-B03C-PP36
GenBank Accession LN502599 [GenBank]
Sequencing primer gene specific primer, PP36: 5'-GCCCTTTAACAATAAACGAATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K104195-0022-250-B03C-PP36
AAAAAGAATTTTTTATCCCAATTTCCCTAAATCATAAAGAAACTTAGGGTTCGTTATCAT
CGCCATCTCGATTCTCCTAAATCATAAGCGATGTTATCTCCGATTGAGAATGAGAGATCC
GAAATTCCTTCCCCTGAGAAGCTGAAGTTAGTGGAGGAGCCATCCGACGATGAATCTAAG
TTCTCTAATTATGATTCGGACGATTGGGAAACTTTGTCAGATGATCTTCGACTGTTAGAT
GAAGAGTGGGGCAAGAGCGGGGTTTGTAATCTCCAACCATTTCTTTCTTTTGAAGAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 53-K104196-0022-250-B03C-8409
GenBank Accession LN508141 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K104196-0022-250-B03C-8409
CGGACTGAGCCTTTACATTCTTCAAAGAAAGAAATGGTTGGAGATTACAAACCCCGCTCT
TGCCCCACTCTTCATCTAACAGTCGAAGATCATCTGACAAAGTTTCCCAATCGTCCGAAT
CATAATTAGAGAACTTAGATTCATCGTCGGATGGCTCCTCCACTAACTTCAGCTTCTCAG
GGGAAGGAATTTCGGATCTCTCATTCTCAATCGGAGATAACATCGCTTATGATTTAGGAG
AATCGAGATGGCGATGATAACGAACCCTAAGTTTCTTTATGATTTAGGGAAATTGGGATA
AAAAATATCTTTTTTTCTTTATTATCGGTTTACCAAAGTTTTCATTCGTTTATGTNTAAA
GGG

Last Updated on 06.12.2018 16:11