Sequences from confirmation amplicon


Confirmation sequences for line 250D06 and hit At2g47250 are shown.
Line ID 250D06
Locus At2g47250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19400593
Primers used for PCR 04ht - 8474, show primer details
   
Sequence Name 33-K112586-0022-250-D06C-04ht
GenBank Accession LN501059 [GenBank]
Sequencing primer gene specific primer, 04ht: 5'-AGTGCGTATGAAATTCCGAGTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112586-0022-250-D06C-04ht
GCAATTAGATGGATGCAAGTGAACCACCTGCCACCAGAATTTACAGGCAACCCGTCAGAT
GTAAGCTATAAGGATCTCTTTATAATCGGGAAATCAAAATCTGTAACAGAATGCAAAAAA
CAAGACATACTTGGTTATCTTTGACGGTCAAGTAATGGCCAGTGCGTTCTAGGTGAGCCA
CCTGCATAAAATAACCAGCAAGCATGGCCTTTCTTATGTTGACATAGTAGTCGCGGCTGT
TGAAATCCGTGCTGCACATCTTCAGATTAAACCTCGACATTATCCGCACTAGCTGCTGTC
TGACATTGTCTGCAGATTTCATTGCTCTATTGTTGACAAAGTTCTCGAAACACCAGTTTG
GGTATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCACACGATTATT
   
Sequence Name 07-K112585-0022-250-D06C-8409
GenBank Accession LN489344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112585-0022-250-D06C-8409
TCGGATGAGCATTTCATTGAATATATCCTGAATACCCAAACTGGTGTTTCGAGAACTTTG
TCACAATAGAGCAATGAAATCTGCAGACAATGTCAGACAGCAGCTAGTGCGGATAATGTC
GAGGTTTAATCTGAAGATGTGCAGCACGGATTTCAACAGCCGCGACTACTATGTCAACAT
AAGAAAGGCCATGCTTGCTGGTTATTTTATGCAGGTGGCTCACCTAGAACGCACTGGCCA
TTACTTGACCGTCAAAGATAACCAAGTATGTCTTGTTTTTTGCATTCTGTTACAGATTTT
GATTTCCCGATTATAAAGAGATCCTTATAGCTTACATCTGACGGGTTGCCTGTAAATTCT
GGTGGCAGGTGGTTCACTTGCATCCATCTAATTGCCTGGATCACAAACCAGAGTGGGTGA
TTTACAATGAATATGTCTTGACTACTCGGAATTTCATACGCAC

Last Updated on 06.12.2018 16:11