Sequences from confirmation amplicon


Confirmation sequences for line 250E06 and hit At4g27230 are shown.
Line ID 250E06
Locus At4g27230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13638469
Primers used for PCR HF35 - 8474, show primer details
   
Sequence Name 34-K032416-022-250-E06C-8409
GenBank Accession LN501303 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032416-022-250-E06C-8409
GAGCCTTTACAATTGAATATATCCTGACGGGTTTCGCTGATACTCATTATTGTTATTTTT
GACAGAGAGAAATTTCTCAGTTACGCTTCATCCTCCTCTAAGAGATCTTTTTTCTATCTT
GGGTAGTAGAGAGAAATGGCGGGTCGGGGAAAACAACTTGGATCTGGTGCAGCGAAGAAG
TCTACTTCTCGTAGTAGCAAGGCTGGGCTTCAATTCCCTGTTGGTCGTATCGCTCGATTT
TTGAAAGCCGGTAAGTACGCCGAGCGTGTTGGTGCCGGAGCTCCGGTCTATCTCGCCGCC
GTTCTTGAATACCTCGCCGCTGAGGTAATCAGTCTCTTCTATTTATCACCTGTTTAATTT
ACTCTTTTTACCGAATTAAATGGTTATAGCTTGCATCTAGGGTTCTGGATTTTAGATTTT
CTTACCCCTTTCGTTAAATTATGCGAAATTTGGAATATTTTAGAATGCATTAGTTCCTTA
GTTTGTTTTTTTCTTGGGAAAAATTGTCCATTTTTTTTGTGTAGTTTTGAGCTCAATTTG
TGTTTCTTTGTGCTCATCATGCTTATCGAAATTAGGGTTAAATTTGTTCCTTACTACTTT
GAGTTATCATAGTTGGCACTGATTGATACTGTCAATTGT
   
Sequence Name 34-K032416-022-250-E06C-HF35
GenBank Accession LN501304 [GenBank]
Sequencing primer gene specific primer, HF35: 5'-TTCGAATTTGAGAACACAATTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032416-022-250-E06C-HF35
GTAGTAAGGAACAAATTTAACCCTAATTTCGATAAGCATGATGAGCACAAAGAAACACAA
ATTGAGCTCAAAACTACACAAAAAAAATGGACAATTTTTCCCAAGAAAAAAACAAACTAA
GGAACTAATGCATTCTAAAATATTCCAAATTTCGCATAATTTAACGAAAGGGGTAAGAAA
ATCTAAAATCCAGAACCCTAGATGCAAGCTATAACCATTTAATTCGGTAAAAAGAGTAAA
TTAAACAGGTGATAAATAGAAGAGACTGATTACCTCAGCGGCGAGGTATTCAAGAACGGC
GGCGAGATAGACCGGAGCTCCGGCACCAACACGCTCGGCGTACTTACCGGCTTTCAAAAA
TCGAGCGATACGACCAACAGGGAATTGAAGCCCAGCCTTGCTACTACGAGAAGTAGACTT
CTTCGCTGCACCAGATCCAAGTTGTTTTCCCCGACCCGCCATTTCTCTCTACTACCCAAG
ATAGAAAAAAGATCTCTTAGAGGAGGATGAAGCGTAACTGAGAAATTTCTCTCTGTCAAA
AATAACAATAATGAGTATCAGCGAAACCCGTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACT
Primers used for PCR FN30 - 8474, show primer details
   
Sequence Name 51-K035338-022-250-E06C-8409
GenBank Accession LN507418 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K035338-022-250-E06C-8409
TTTCTGACAATGATATGACACTGCATGGATCGATAATCTACACCGTCAGATTGTTACAAG
TTTAAGGGACGTGATTTGAGATTCGTGCGCCCACCTGCTTTGTCCTTTTTTTGGTTCTCC
GATGATTTCAATGGATTGTTATATGGGCTATATTGGGCTTGTTAAATAGAAATACTCCTT
TTTATTTTAACGTTATCTGGCCCAAAATTTAACACTGTTCGGCCCAAATAAATTGTTTCT
TCACTTCTTGTACATATGTATTTTCCTTTTTATCAAAATTTCAAAGAAGTTAGGGAGAAG
TTATAACTTACTAAGTGTTGTAATGATTATTGTTTGCTTTTTAGACGTATTCTTTATGTG
GGTTTAAATTTGGATAATAACAGTTGCAAGTTGTATGACATATTGTGGTGGCTGAAAAGC
CCACAAATCTTCTCAGTTAGTAAGAACTCAAAAGTTGTAATATTAATTTAGACAACGTTA
CTTTCGTTGTTATCCATCTCCGGAGAAAACGTTACAATTTGTCAAAAAAGTATTAATGTC
AAGCTAATTT
   
Sequence Name 51-K035338-022-250-E06C-FN30
GenBank Accession LN507419 [GenBank]
Sequencing primer gene specific primer, FN30: 5'-CATCTTCGGAGAAATTAGCTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K035338-022-250-E06C-FN30
GAAATTGTACGTTTTCTCCGGAGATGGATAACAACGAAAGTAACGTTGTCTAAATTAATA
TTACAACTTTTGAGTTCTTACTAACTGAGAAGATTTGTGGGCTTTTCAGCCACCACAATA
TGTCATACAACTTGCAACTGTTATTATCCAAATTTAAACCCACATAAAGAATACGTCTAA
AAAGCAAACAATAATCATTACAACACTTAGTAAGTTATAACTTCTCCCTAACTTCTTTGA
AATTTTGATAAAAAGGAAAATACATATGTACAAGAAGTGAAGAAACAATTTATTTGGGCC
GAACAGTGTTAAATTTTGGGCCAGATAACGTTAAAATAAAAAGGAGTATTTCTATTTAAC
AAGCCCAATATAGCCCATATAACAATCCATTGAAATCATCGGAGAACCAAAAAAAGGACA
AAGCAGGTGGGCGCACGAATCTCAAATCACGTCCCTTAAACTTGTAACAATCTGACGGTG
TAGATTATCGATCCATGCAGTGTCATATCATTGGTCAGAAATATTTTCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCCACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11