Sequences from confirmation amplicon


Confirmation sequences for line 250F03 and hit At3g51440 are shown.
Line ID 250F03
Locus At3g51440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19089656
Primers used for PCR AK35 - 8474, show primer details
   
Sequence Name 08-K105632-0022-250-F03C-AK35
GenBank Accession LN489729 [GenBank]
Sequencing primer gene specific primer, AK35: 5'-TCACTCATCTCCTCTACTGCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K105632-0022-250-F03C-AK35
TCTCACCGGAGCTGAGTTATCGGAGTCGGTCTTCTCATAGCCCCGAAGACATCGCCTACC
ATGAAGACTCTGGTTTCATCTACACTGGTTGCGTTGATGGATGGGTCAAACGAGTCAAGG
TCGCCGAGTCGGTTAATGACTCACTCGTTGAAGATTTGGTCAACACCGGTGGTAGACCGC
TCGGAATCGCTTTCGGAATCCACGGCGAAGTCATCGTCGCTGACGCTTACAAGGTTTGGG
TTCGTGACTTTTTTGAAATCGAAGTTTGAAAATTTTCAATTAATAGGTTGGTTTCTGTCT
GGGTAGGGACTGTTGAATATAAGCGGTGACGGGAAGAAGACGGAATTGTTAACGGAAGAA
GCGGACGGCGTTAGATTTACCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 24-K105633-0022-250-F03C-8409
GenBank Accession LN496990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105633-0022-250-F03C-8409
GCCTTGGTAATCTAACGCCGTCCGCTTCTTCCGTTAACAATTCCGTCTTCTTCCCGTCAC
CGCTTATATTCAACAGTCCCTACCCAGACAGAAACCAACCTATTAATTGAAAATTTTCAA
ACTTCGATTTCAAAAAAGTCACGAACCCAAACCTTGTAAGCGTCAGCGACGATGACTTCG
CCGTGGATTCCGAAAGCGATTCCGAGCGGTCTACCACCGGTGTTGACCAAATCTTCAACG
AGTGAGTCATTAACCGACTCGGCGACCTTGACTCGTTTGACCCATCCATCAACGCAACCA
GTGTAGATGAAACCAGAGTCTTCATGGTAGGCGATGTCTTCGGGGCTATTGAGAAGACCG
ACTCCGATAAACTCAGCTCCGGTGAGAAATCTTTCGTTTATTAGAGGCGGGATTGAAGCA
GTAGAGAGAATGAGTGA

Last Updated on 06.12.2018 16:11