Sequences from confirmation amplicon


Confirmation sequences for line 250G03 and hit At5g08750 are shown.
Line ID 250G03
Locus At5g08750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2854315
Primers used for PCR KM62 - 8474, show primer details
   
Sequence Name 73-K101221-0022-250-G03C-8409
GenBank Accession LN512857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101221-0022-250-G03C-8409
GTTCACACGCTTCTCACTCCTCCCATGATAAATTCTCCATCATCCTTCAAGACACAAAAT
GTACTCTTTTTGGTCTCTGTCAATATCATGTACGTTATTCTCTTTCAAGACACAAAATGT
ACAATTAATTACTCTTATCAAAAAGAAAAAAAGGGGTGAAACTGAATGTTATCATCTCAC
AACAAAGTCTCTTGGTCGTATTTATTTTTGTATTATGTTAGTTTAGCAAAAAAATTCATC
TCACAACAAAGTCTCAAGTTTTTACGTAACACGCGAAAGTATTCATGATTGCCTCTTTTA
TTTTTGTGTCATTGTGCTTGCATTTCTTAGAGAGAAAAAAAAGTGTAAGATACTGTGTTC
AATTTTGAAGATTGGCTAAACAATTTAACTAAAAGACACAATTATTTATGTTTGGAACCA
TTAGACTCGACACTGTATCAAAACTAGAAAACAAGATCCAAAGTCTTTAACATTTGTTGG
ATAGCAAAATAATGGTAGATATTAAGTCATTAGTCAGTGTACAAGGCAGAAGCGCGTTAA
CTTGGAATTTAGTTGGTGAGTCCGATGATTCACTCTATTTCTGTATATATACAGACCATT
TGATGCATTTTAACAAAGAGAATCAGTTAATATGTCTAAAGAGAAATCATATGTAATTGC
TCTTCTTCTATCTNTGCTTCTCTGTCTTTCTTTTCAAG
   
Sequence Name 73-K101228-0022-250-G03C-KM62
GenBank Accession LN512858 [GenBank]
Sequencing primer gene specific primer, KM62: 5'-TCAGAGACACCAACTTGAAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101228-0022-250-G03C-KM62
GAGCATTCATATGATTTCTCTTTAGACATATTAACTGATTCTCTTTGTTAAAATGCATCA
AATGGTCTGTATATATACAGAAATAGAGTGAATCATCGGACTCACCAACTAAATTCCAAG
TTAACGCGCTTCTGCCTTGTACACTGACTAATGACTTAATATCTACCATTATTTTGCTAT
CCAACAAATGTTAAAGACTTTGGATCTTGTTTTCTAGTTTTGATACAGTGTCGAGTCTAA
TGGTTCCAAACATAAATAATTGTGTCTTTTAGTTAAATTGTTTAGCCAATCTTCAAAATT
GAACACAGTATCTTACACTTTTTTTTCTCTCTAAGAAATGCAAGCACAATGACACAAAAA
TAAAAGAGGCAATCATGAATACTTTCGCGTGTTACGTAAAAACTTGAGACTTTGTTGTGA
GATGAATTTTTTTGCTAAACTAACATAATACAAAAATAAATACGACCAAGAGACTTTGTT
GTGAGATGATAACATTCAGTTTCACCCCTTTTTTTCTTTTTGATAAGAGTAATTAATTGT
ACATTTTGTGTCTTGAAAGAGAATAACGTACATGATATTGACNGAGACCAAAAAGAGTAC
ATTTTGTGTCTTGAAGGATGATGGAGAATTTATCATGTGGAGGAGTGAGAAGCGTGTGAA
CGTGAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT

Last Updated on 06.12.2018 16:11