Sequences from confirmation amplicon


Confirmation sequences for line 251F09 and hit At3g17810 are shown.
Line ID 251F09
Locus At3g17810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6095427
Primers used for PCR RZ09 - 8474, show primer details
   
Sequence Name 15-K017722-022-251-F09C-RZ09
GenBank Accession LN492753 [GenBank]
Sequencing primer gene specific primer, RZ09: 5'-CCTTATAAGAGTATCCCCCTGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017722-022-251-F09C-RZ09
AACTGCCAAACACTATCAACGTTAGCTATTCTTGTAATGCCATTACAAGATTTGCCAAAA
CGTTTGACATTATAAAAGTGCTATCTAGCTAACCCTTCAACGCATGGCTCAGGACGCAAC
GTCTTCATATCAATTCCCATTACACTCATGATTGTATTGATCGCTGCAATTCCTTCACAT
CCGGATTTTAGTGACACCCTAGCAGGCTATTCAAAAGAAAAAGCAAAACGGACATCAAAA
ATGAAAGTAGGGAGCTTTTCAATAAACTTGCATCAAAGTTTTTCAATAAAATGTAGGCAG
CAAATCTAATGTAGAAAAACCAATATGGATCAAATAATTACCAATTTTAAATCGAAGTTT
CATGTCCTAAGAGAAAAGAGCGTTTATTAGAATAATCGGATATTTAAAAGGGCGTGAAAA
GGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGAGTGC
TTGGCATTCCGTGCGATAATGACTTAGGTTTCATGTCCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 16-K017722-022-251-F09C-8409
GenBank Accession LN493220 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K017722-022-251-F09C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACATGAAA
CCTAAGTCATTATCGCACGGAATGCCAAGCACTCCCGAGGGGAACCCTGTGGTTGGCATG
CACATACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCGATTATTCT
AATAAACGCTCTTTTCTCTTAGGACATGAAACTTCGATTTAAAATTGGTAATTATTTGAT
CCATATTGGTTTTTCTACATTAGATTTGCTGCCTACATTTTATTGAAAAACTTTGATGCA
AGTTTATTGAAAAGCTCCCTACTTTCATTTTTGATGTCCGTTTTGCTTTTTCTTTTGAAT
AGCCTGCTAGGGTGTCACTAAAATCCGGATGTGAAGGAATTGCAGCGATCAATACAATCA
TGAGTGTAATGGGAATTGATATGAAGACGTTGCGTCCTGAGCCATGCGTTGAAGGGTTAG
CTAGATAGCACTTTTATAATGTCAAACGTTTTGGCAAATCTTGTAATGGCATTACAAGAA
TAGCTAACGTTGATAGTGTTTTGCAGTTACTCCACTCCAGGGGGATA

Last Updated on 06.12.2018 16:11