Sequences from confirmation amplicon


Confirmation sequences for line 252F08 and hit At1g51870 are shown.
Line ID 252F08
Locus At1g51870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19265943
Primers used for PCR PP15 - 8474, show primer details
   
Sequence Name 12-K102723-0022-252-F08C-8409
GenBank Accession LN491527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K102723-0022-252-F08C-8409
GCCATTTACATTCAATATATCCCGTCAATTGAATTAAAAGACAAGAATGAGTTAAGCAAA
AGCTTCCCTTTAACCCCACTCCCTCTTTGAGAATTAGGTTAATTGTTGGAGTTTAATTCT
TTATTAATCATCAGAAAGAATTATACCTAAACCTAATGTCAAAAAGAAAAATCACCAACC
CTAAACCTAATGTCAAAAAGAAATACGTAATTGCTATTAAATTAAAAAGAAACCACCAAA
ACAAATTAATTTAAACGTTCTAAGACTACCCTAATCATCCTAACCAGTCTTGTATCACAC
ACAAAGAACAAAACGAAGACAAAAATTATGGTTGCCTAACAAGATTTATTATTTCTTACC
TGACGATAGATGTGGAATCAG
   
Sequence Name 28-K102728-0022-252-F08C-PP15
GenBank Accession LN498795 [GenBank]
Sequencing primer gene specific primer, PP15: 5'-TCTGATTCAACATCTATCGTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K102728-0022-252-F08C-PP15
GCACATAATTTTTGTCTTCGTTTTGTTCTTTGTGTGTGATACAAGACTGGTTAGGATGAT
TAGGGTAGTCTTAGAACGTTTAAATTAATTTGTTTTGGTGGTTTCTTTTTAATTTAATAG
CAATTACGTATTTCTTTTTGACATTAGGTTTAGGGTTGGTGATTTTTCTTTTTGACATTA
GGTTTAGGTATAATTCTTTCTGATGATTAATAAAGAATTAAACTCCAACAATTAACCTAA
TTCTCAAAGAGGGAGTGGGGTTAAAGGGAAGCTTTTGCTTAACTCATTCTTGTCTTTTAA
TTCAATTGACGGGATATATTGAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11