Sequences from confirmation amplicon


Confirmation sequences for line 252F11 and hit At1g28050 are shown.
Line ID 252F11
Locus At1g28050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9776466
Primers used for PCR 09nt - 8474, show primer details
   
Sequence Name 71-K114626-0022-252-F11C-09nt
GenBank Accession LN512604 [GenBank]
Sequencing primer gene specific primer, 09nt: 5'-CAATATGCTCTGTGAAATGCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114626-0022-252-F11C-09nt
CAAGGTAATTGGACGATTGTTGCTCTCAGATTTTGTTGGTTGTACCTGGCCTGAAGTTGA
TCGCTTGTAGTCATCCTGCAAAAGAATCCTAATCAGCATCACAGTTAAGTTGCATAGCAG
TCTAGCAAATATATATCATAAGCCAAAATGTTAGAATTGGTGGCTTGGTGATGAGGTAAA
ACAAAGAAAAGCAAACCAGCTTGTAACTTTTTTCTGACTAGGTTTCATGTTCTGAATTCT
ATCATTCACTAAAAGAAGCAACTGTCTACAACAATTTTTGAGGATGAACGGGAGATGACA
ACGTATTGTGTTTTAAACTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCACACGATTATA
   
Sequence Name 71-K114625-0022-252-F11C-8409
GenBank Accession LN512603 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114625-0022-252-F11C-8409
AAACACAATACGTTGTCATCTCCCGTTCATCCTCAAAAATTGTTGTAGACAGTTGCTTCT
TTTAGTGAATGATAGAATTCAGAACATGAAACCTAGTCAGAAAAAAGTTACAAGCTGGTT
TGCTTTTCTTTGTTTTACCTCATCACCAAGCCACCAATTCTAACATTTTGGCTTATGATA
TATATTTGCTAGACTGCTATGCAACTTAACTGTGATGCTGATTAGGATTCTTTTGCAGGA
TGACTACAAGCGATCAACTTCAGGCCAGGTACAACCAACAAAATCTGAGAGCAACAATCG
TCCAATTACCTTTGGCTCTGAGAAAGGTTCGAACTCCTCCNGTGACTTGCATTTCAAGAG
NCATAT

Last Updated on 06.12.2018 16:11