Sequences from confirmation amplicon


Confirmation sequences for line 252G10 and hit At5g02320 are shown.
Line ID 252G10
Locus At5g02320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:483867
Primers used for PCR RS03 - 8474, show primer details
   
Sequence Name 13-K018613-022-252-G10C-RS03
GenBank Accession LN491831 [GenBank]
Sequencing primer gene specific primer, RS03: 5'-ACCTGTACTTTTTGCAGGACTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K018613-022-252-G10C-RS03
ATAAAGAACCATTGGAATAGTTCACTGAAGAAAAAATTAGAGTTCTACTTAGCAACTGGT
AACTTGCCACCGCCAGCAAGCAAATTCATCGTTCTGAAAGATATTGCTGATGGAGACAGA
GATTCCAAGCAGTCTAGTGCAACTAAACCTTTCAAGGATTCTGATTCGCTTACCCAAACC
TCTTCAGGAAATACAGATTCAAATGAAGTTGGAAGAGACCATTTTGACTCTTCTTCCGCT
CTGCTTGAAGAAGTAGCTGCTTCACGAAGAATCGGTGTCAATGAGTATGCTTGCTCTCCT
GTTGAGTACAAGCCTCAGTTGCCTAATCTGGAACCAATTTCAGAGGAAGTCAGAATCAAC
TCAAAAGCTTATTTTGAGAGATCAATCCAGAGGAAAGTAGAAAACGGGTTTGGAACTCCA
AAGCATGGGAATTTATACTATAAGTCTCCACTTGACTATTACTTTCCATCAGAAGCGGAT
CTGCAACATATGTACGGATATGAATGTGGTTGCAGTCCCGGTGCTGCATCACCAGTCAGC
TTAATGACTACACCCTGTAATAAAGACAGTGGTTTAACTGTCTACTTTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTT
   
Sequence Name 14-K018613-022-252-G10C-8409
GenBank Accession LN492298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K018613-022-252-G10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAAAGTAGACAGTTAAACCACTGTCTTTATTAC
AGGGTGTAGTCATTAAGCTGACTGGTGATGCAGCACCGGGACTGCAACCACATTCATATC
CGTACATATGTTGCAGATCCGCTTCTGATGGAAAGTAATAGTCAAGTGGAGACTTATAGT
ATAAATTCCCATGCTTTGGAGTTCCAAACCCGTTTTCTACTTTCCTCTGGATTGATCTCT
CAAAATAAGCTTTTGAGTTGATTCTGACTTCCTCTGAAATTGGTTCCAGATTAGGCAACT
GAGGCTTGTACTCAACAGGAGAGCAAGCATACTCATTGACACCGATTCTTCGTGAAGCAG
CTACTTCTTCAAGCAGAGCGGAAGAAGAGTCAAAATGGTCTCTTCCAACTTCATTTGAAT
CTGTATTTCCTGAAGAGGTTTGGGTAAGCGAATCAGAATCCTTGAAAGGTTTAGTTGCAC
TAGACTGCTTGGAATCTCTGTCTCCATCAGCAATATCTTTCAGAACGATGAATTTGCTTG
CTGGCGGTGGCAAGTTACCAGTTGCTAAGTAGAACTCTCATTTTTTCTTCAGTGAACTAT
TCCNATGGTTCTTTATGGCGTTATCAGTCCTG

Last Updated on 06.12.2018 16:11