Sequences from confirmation amplicon


Confirmation sequences for line 252H10 and hit At5g02380 are shown.
Line ID 252H10
Locus At5g02380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:507020
Primers used for PCR 09up - 8474, show primer details
   
Sequence Name 33-K117403-0022-252-H10C-09up
GenBank Accession LN501162 [GenBank]
Sequencing primer gene specific primer, 09up: 5'-TTGGAAAAGGGCTGTAAACTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117403-0022-252-H10C-09up
ACGGTCTACAAATTCTAATTTTGCCTATAAATATCAAAGCTCCTGAATATGTAAGTTTCA
TTCACTGATTATCGTTTAAGGCAAATTAAGATCATCTTCATAAATCTTCTCAGATCTCTT
CCAATTTTCTAGAAAAAACATGTCTTGCTGTGGTGGAAGCTGTGGTTGTGGATCTGCCTG
CAAGTGCGGCAATGGTTGCGGAGGGTACGCGTTTCTCGATCTCTCATAATTCTGATTCTT
CTGGTGTTTCTTGTGATTTTCTGAATCAAAAACCCTAATTTCTCCATATTTACAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 09-K117402-0022-252-H10C-8409
GenBank Accession LN490373 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117402-0022-252-H10C-8409
GAGAAATTAGGGTTTTTGATTCAGAAAATCACAAGAAACACCAGAAGAATCAGAATTATG
AGAGATCGAGAAACGCGTACCCTCCGCAACCATTGCCGCACTTGCAGGCAGATCCACAAC
CACAGCTTCCACCACAGCAAGACATGTTTTTTCTAGAAAATTGGAAGAGATCTGAGAAGA
TTTATGAAGATGATCTTAATTTGCCTTAAACGATAATCAGTGAATGAAACTTACATATTC
AGGAGCTTTGATATTTATAGGCAAAATTAGAATTTGTAGACCGTCCACGTGTCAACGATC
TTTGTCCACTCTTATCACCTAAATAATCGTTCCTCAAGTAAATAATTTTAGTTTACAGCC
CTTTTTCCAA

Last Updated on 06.12.2018 16:11