Sequences from confirmation amplicon


Confirmation sequences for line 253C04 and hit At5g22920 are shown.
Line ID 253C04
Locus At5g22920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7666619
Primers used for PCR 07ed - 8474, show primer details
   
Sequence Name 17-K110172-0022-253-C04C-07ed
GenBank Accession LN493895 [GenBank]
Sequencing primer gene specific primer, 07ed: 5'-CAAGATATGGTTGTTTTGGGCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K110172-0022-253-C04C-07ed
AGCAGCTTTTGAATCTGTGATGATATAATTGTTAACAACCAAAGGCATTGACTGATACCA
AACTATAGAAAGTACAAATAATCTCCTCATGGGAAATTCAGACACCAAAACCAGCTCTGG
TTAAAAATTAACCGGTTGAACCAACAACCTGAGGCATTCCAGAGGAGCAAGAGTGACTAT
CACAGCCTCTCTGTGTCTGTCTTGTATTGTAGGAACCACAGCTACTGCATTTATGCGCGA
TCAAATGAAACCGAACGTTCGTGTTTGAGCCGCAGTCATTGCAGAGAATCCATACCTGAC
TCAACACATCACATTTAAATCCAAATCAGAAAGTGTACATAACACAAACACACACACACA
CACGAGAATCACATATCGAATTTAATTTTGGTTTACCATCTCGTTTTCATACATCTATGG
CATTGGGTATGCAGCAACCTGCAAAATTTTGAATGTAAGACTTAGTGAAACATAAGAGAC
TATACGAGGAGACCGAACATCAGATCAAGAAACCTCACCTCTTCATCAAGTTTCTTCCAC
ATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGC
AGCGTT
   
Sequence Name 01-K110171-0022-253-C04C-8409
GenBank Accession LN486619 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K110171-0022-253-C04C-8409
GTGGAAGAACTTGATGAAGAGGTGAGGTTTCTTGATCTGATGTTCGGTCTCCTTGTATAG
TCTCTTATGTTTCACTAAGTCTTACATTCAAAATTTTGCAGGTTGCTGCATACCCAATGC
CAAAGATGTATGAAAACAAGATGGTAAACCAAAATTAAATTCGATATTTGATTCTCGTGT
GTGTGTGTGTGTTTGTGTTATGTACACTTTCTGATTTGGATTTAAATGTGATGTGTTGAG
TCAGGTATGGATTCTCTGCAATGACTGCGGCTCAAACACGAACGTTCGGTTTCATTTGAT
CGCGCATAAATGCAGTAGCTGTGGTTCCTACAATACAAGACAGACACAGAGAGGCTCTGA
TAGTCACTCTTGCTCCTCTGGAATGCCTCAGGTTGTTGGTTCAACCGGTTAATTTTTAAC
CAGAGCTGGTTTTGGTGTCTGAATTTCCCATGAGGAGATTATTTGTACTTTCTATAGTTT
GGTATCAGTCAATGCCTTTGGTTGTTAACAATTATATCATCACAGATTCAAAAGCTGCTT
TGAGCCAATCCAACTTGCAAGTCTAGACCATTATAGCCCAAAACAACCATATCT

Last Updated on 06.12.2018 16:11