Sequences from confirmation amplicon


Confirmation sequences for line 254B04 and hit At2g18450 are shown.
Line ID 254B04
Locus At2g18450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7997811
Primers used for PCR AU06 - 8474, show primer details
   
Sequence Name 09-K106284-0022-254-B04C-8409
GenBank Accession LN490188 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106284-0022-254-B04C-8409
TTACTCTTTTCGTATCCACATTTCGACTAAGACAATTTTTGTTGTCTTAAGGAAAATAGA
ACTAACCGTGAAATCCTCACGAGCATGTGCTCCTCGACTCTCCTTCCTAGCCTCAGCAGA
ATGCATTGTTATGCATGCATTAACCAAAAGATTCTCTAGCTCCATAGTTTCTATCAAATC
CGAGTTCCTAAAATGCAAAGTTTCCAATTACATAAACTTTAGTAATTAGCCTAAAGACTA
GCTTTTCAACGTTTTAAAGTAAAGTCTCACCATATCATACTCCGATCAGTCACTTTGACA
TCACCAAAACTGTCCCACGTCTTATCAATCAAATCACAACCTATAATCATAACCTTTTAC
CAATAAATTCACTTTAAAACTTCTTGAGTTCTTATCATACGGTAGAGTTTCAGTGTTGGT
TACCTTCCTCTAGTGTTTCTTGTGTACGGAAAACAGCAGCATTGTTTTGCATTACTCTTT
GCATGTTTAAGCGATTTTTTAGAAG
   
Sequence Name 09-K106285-0022-254-B04C-AU06
GenBank Accession LN490189 [GenBank]
Sequencing primer gene specific primer, AU06: 5'-CTTCTAAAATCCGCTTAAACATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106285-0022-254-B04C-AU06
TGCTGCTGTTTTCCGTACCAGAAACACTAGAGGAAGGTAACCAACACTGAAACTCTACCG
TATGATAAGAACTCAAGAAGTTTTAAAGTGAATTTATTGGTAAAAGGTTATGATTATAGG
TTGTGATTTGATTGATAAGACGTGGGACAGTTTTGGTGATGTCAAAGTGACTGATCGGAG
TATGATATGGTGAGACTTTACTTTAAAACGTTGAAAAGCTAGTCTTTAGGCTAATTACTA
AAGTTTATGTAATTGGAAACTTTGCATTTTAGGAACTCGGATTTGATAGAAACTATGGAG
CTAGAGAATCTTTTGGTTAATGCATGCATAACAATGCATTCTGCTGAGGCTAGGAAGGAG
AGTCGAGGAGCACATGCTCGTGAGGATTTCACGGTTAGTTCTATTTTCCTTAAGACAACA
AAAATTGTCTTAGTCGAAATGTGGATAACGAAAAGAGTAAATTATTCAGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTA
GTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11