Sequences from confirmation amplicon


Confirmation sequences for line 254F03 and hit At1g12320 are shown.
Line ID 254F03
Locus At1g12320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4189445
Primers used for PCR 15sa - 8474, show primer details
   
Sequence Name 35-K116569-0022-254-F03C-15sa
GenBank Accession LN501971 [GenBank]
Sequencing primer gene specific primer, 15sa: 5'-TGCATTCTCTATGTCCATTTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116569-0022-254-F03C-15sa
ATCGGGGGGTACTTGATTAAGTTGATAGTATTGGGGCTAAACCTAAAAACTAGACAAATA
TACAAGGGCCATATCGATACAAAACGAAACGAGACTTTTCTTAATGTCTACGTTCTCTAT
AAAAGCAAACTTTAAGCTAAAACCCAAACAAGAAGCAGAACACAAGAAACGTGAGAAACT
TTGAGAAGACCAAGAAGAAGAAGCAGAAGAAGAAGAAAATGAAATTGGTTTGGTCTCCGG
GAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGGGATTATTAT
   
Sequence Name 06-K116568-0022-254-F03C-8409
GenBank Accession LN488977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116568-0022-254-F03C-8409
TCGGATGAGCATTTCATTGAATATATCCTGCTTCCCGGAGACCAAACCAATTTCATTTTC
TTCTTCTTCTGCTTCTTCTTCTTGGTCTTCTCAAAGTTTCTCACGTTTCTTGTGTTCTGC
TTCTTGTTTGGGTTTTAGCTTAAAGTTTGCTTTTATAGAGAACGTAGACATTAAGAAAAG
TCTCGTTTCGTTTTGTATCGATATGGCCCTTGTATATTTGTCTAGTTTTTAGGTTTAGCC
CCAATACTATCAACTTTAATCAAGTACCCCCCGATTTAGCTCCTTTGTTTAGAAATTGTT
GCTTTAAAATCAGAAAATGGACATANAGAATGC

Last Updated on 06.12.2018 16:11