Sequences from confirmation amplicon


Confirmation sequences for line 254G01 and hit At5g46700 are shown.
Line ID 254G01
Locus At5g46700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18951376
Primers used for PCR EO01 - 8474, show primer details
   
Sequence Name 25-K018475-022-254-G01C-EO01
GenBank Accession LN497233 [GenBank]
Sequencing primer gene specific primer, EO01: 5'-TACCATTACCCTAAATCCCATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K018475-022-254-G01C-EO01
AAAACATATTTATAAAACATTAATCACTTTTCACACATGTGAATCTTTTTCTTTTTCTGT
CTCTCTTTAAGTTCAATTCTATTTCTCTCTCTATTTCAAACCAAACAGATTTCAAGAACA
CAAAAACAGAGTTAAAACTCTCATCTCTCCCAAAAAAGATGCCTTTAAGCAACAATGTAA
TTGGTTGCATAAACTTCATCACCGTCCTCCTCTCCATTCCGGTCATCGGCGCCGGAATCT
GGCTAGCCATAGGAACAGTAAACTCATGCGTCAAGCTTCTTCAATGGCCAGTAATAATCC
TCGGAGTCTTAATCCTCTTAGTGGGTCTCGCTGGTTTCATTGGAGGGTTTTGGAGAATCA
CATGGCTTCTTGTTGTTTACTTAATCGCCATGCTTATTCTCATTGTACTTTTGGGTTGCC
TTGTCGGATTTATTTACATGGTTACCATAAGAGGCTCTGGTCATCCAGAACCAAGTAGAG
CTTATCTTGAGTATAGTCTTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTTTTATAAAATGAAAGTACATTTTGATAAAACGACNA
ATTACGATCCGTCGTATTTATTGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTT
   
Sequence Name 26-K018475-022-254-G01C-8409
GenBank Accession LN497708 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K018475-022-254-G01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTATAAGACTATACTCAAGATAAGCTCTACTT
GGTTCTGGATGACCAGAGCCTCTTATGGTAACCATGTAAATAAATCCGACAAGGCAACCC
AAAAGTACAATGAGAATAAGCATGGCGATTAAGTAAACAACAAGAAGCCATGTGATTCTC
CAAAACCCTCCAATGAAACCAGCGAGACCCACTAAGAGGATTAAGACTCCGAGGATTATT
ACTGGCCATTGAAGAAGCTTGACGCATGAGTTTACTGTTCCTATGGCTAGCCAGATTCCG
GCGCCGATGACCGGAATGGAGAGGAGGACGGTGATGAAGTTTATGCAACCAATTACATTG
TTGCTTAAAGGCATCTTTTTTGGGAGAGATGAGAGTTTTAACTCTGTTTTTGTGTTCTTG
AAATCTGTTTGGTTTGAAATAGAGAGAGAAATAGAATTGAACTTAAAGAGAGACAGAAAA
AGAAAAAGATTCACATGTGTGAAAAGTGATTAATGTTTTATAAATATGTTTTTGGTTTGA
TGGGATT

Last Updated on 06.12.2018 16:11