Sequences from confirmation amplicon


Confirmation sequences for line 255B01 and hit At4g27070 are shown.
Line ID 255B01
Locus At4g27070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13588472
Primers used for PCR MR90 - 8474, show primer details
   
Sequence Name 51-K017462-022-255-B01C-MR90
GenBank Accession LN507345 [GenBank]
Sequencing primer gene specific primer, MR90: 5'-TAAAGAAACAAGGAAACAAGCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K017462-022-255-B01C-MR90
ATGGGGCGGGAAGCTGATGTTCTCGTTGCTTGTGTCGGTGGTGGTTCAAATGCTATGGGA
CTCTTCCATGAATTTGTCGATGACACAGAGGTTCGAATGATTGGTGTGGAAGCCGCTGGA
TTTGGATTGGACAGCGGCAAACATGCTGCCACATTGACAAAAGGAGATGTCGGTGTACTC
CACGGAGCTATGAGTTACTTGCTGCAAGATGATGATGGCCAAATCATTGAACCACACTCC
ATCAGTGCCGGGTAAGAGATTCTTCTCTGTTCAAAGTTGGTTTTAAGCCACATGTATCTG
GTCACCTGTATCTAAAGAGTAAAAGTTGATGTTATCAGATTGGACTACCCTGGAGTCGGA
CCTGAGCATAGTTTCCTTAAAGACGTGGGAAGGGCTGAATACTTTAGCGTTACTGATGAA
GAAGCATTAGAAGGTACTTACTTGACACTTCATTTTTCTCCTTTTAGATGTTTCTTTCAC
AACTCATGAGAACTTGCATTGTGTATTCTAATCTATGCGTGTAGCTTTCAAGAGAGTGTC
TCGGTTGGAGGGATTATTCATGGCTATATTCAATTGACCATATGGCTTTTTCTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCCATTTTTTTTCAAATTCAAAATGTAGA
T
   
Sequence Name 52-K017462-022-255-B01C-8409
GenBank Accession LN507668 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K017462-022-255-B01C-8409
ATGAAGCCCTTTACAATTGAATATATCCCTGAGAAAAAGCCATATGGTCAATTGAATATA
GCCATGAATAATCCCTCCAACCGAGACACTCTCTTGAAAGCTACACGCATAGATTAGAAT
ACACAATGCAAGTTCTCATGAGTTGTGAAAGAAACATCTAAAAGGAGAAAAATGAAGTGT
CAAGTAAGTACCTTCTAATGCTTCTTCATCAGTAACGCTAAAGTATTCAGCCCTTCCCAC
GTCTTTAAGGAAACTATGCTCAGGTCCGACTCCAGGGTAGTCCAATCTGATAACATCAAC
TTTTACTCTTTAGATACAGGTGACCAGATACATGTGGCTTAAAACCAACTTTGAACAGAG
AAGAATCTCTTACCCGGCACTGATGGAGTGTGGTTCAATGATTTGGCCATCATCATCTTG
CAGCAAGTAACTCATAGCTCCGTGGAGTACACCGACATCTCCTTTTGTCAATGTGGCAGC
ATGTTTGCCGCTGTCCAATCCAAATCCAGCGGCTTCCACACCAATCATTCGAACCTCTGT
GTCATCGACAAATTCATGGAAGAGTCCCATAGCATTTGAACCACCACCGACACAAGCAAC
CAGAACATCAGGCTTCCCGCCCCATTTCTCATCCT

Last Updated on 06.12.2018 16:11