Sequences from confirmation amplicon


Confirmation sequences for line 255B06 and hit At5g44550 are shown.
Line ID 255B06
Locus At5g44550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17942902
Primers used for PCR 05xh - 8474, show primer details
   
Sequence Name 55-K119268-0022-255-B06C-05xh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 05xh: 5'-CAAAACATGCATTAAACAGAATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K119268-0022-255-B06C-05xh
CGGATTACCTCTCTACATTAGTTAATTTAGCAATTAATCGATTAGAAAACAATCAACCAA
AAAAATCCTAAACATCATGTAATCAAATGAAATACTTCTTTTAAAAAGTGTGAATAATAC
TAAACTAAAATGATTAAAAAAGTATATGAACGTAACATCTCCAACTAGTTGGTCTATATT
CGTGAACAACTAAAATTAAGACTTTTAGAAGAAAAATAGTAATTTATACAAAATAATACA
TATGTGTGTATATTTATATATTTACCATGTCAAGAATGGCGACTGAGAGAAGCCGAAAAC
CAGTGAACTCCATTTTCCCTCCAAATATCTGAAGAGCAATCATCAACAAGTTGTGGAAGC
TCACCATTGCATTAGCTACTACGAAAAACCTTTTATACACCAAAATCATCAAAAAGATTA
CCAATGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTTATT
   
Sequence Name 31-K119267-0022-255-B06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K119267-0022-255-B06C-8409
TCTTTTTGATGATTTTGGTGTATAAAAGGTTTTTCGTAGTAGCTAATGCAATGGTGAGCT
TCCACAACTTGTTGATGATTGCTCTTCAGATATTTGGAGGGAAAATGGAGTTCACTGGTT
TTCGGCTTCTCTCAGTCGCCATTCTTGACATGGTAAATATATAAATATACACACATATGT
ATTATTTTGTATAAATTACTATTTTTCTTCTAAAAGTCTTAATTTTAGTTGTTCACGAAT
ATAGACCAACTAGTTGGAGATGTTACGTTCATATACTTTTTTAATCATTTTAGTTTAGTA
TTATTCACACTTTTTAAAAGAAGTATTTCATTTGATTACATGATGTTTAGGATTTTTTTG
GTTGATTGTTTTCTAATCGATTAATTGCTAAATTAAACTAATGTAGAGATGGATAATCCG
GTTCATGTTTAGATTCGATTCTGTTTAATGCATGTTTTG

Last Updated on 06.12.2018 16:11