Sequences from confirmation amplicon


Confirmation sequences for line 255B08 and hit At3g61670 are shown.
Line ID 255B08
Locus At3g61670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22820927
Primers used for PCR CK52 - 8474, show primer details
   
Sequence Name 28-K018512-022-255-B08C-CK52
GenBank Accession LN498645 [GenBank]
Sequencing primer gene specific primer, CK52: 5'-CAGGAACATATTGACAAGAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K018512-022-255-B08C-CK52
AAAATGCGTTTACCGGTAATATTCCAAGACCTGGACCTCCCATTACTCCCCAGAATCCAG
CTCTGTAATCATACCTAGAATGTCCCCAAAACACATTAGCAGTCATGAAGAAGATGTCAT
AACAGAAGAGGCATCGAAAGTTACTTACCAGTAGTTTCCTGGCTGAATGACTCCGGCTTG
CTTCTCTGCTTTTCTCAGCAGACGTTCGGTTAAAGGATGTCCATTTATCGAAACATTGCT
TTTGTTCCCTTCATCATTCTGAATAGATTTTGTCAGATCTTTGAAGCTTTTCTTCACAAT
GCTCGCGAATCCGCTCTTCTTAGCTCTTTGCTGCTGATCTTTAGATACTCCACTGTTACG
ATGAGAGTAGTCGTTGAAGTTAACTTCCATCTCACTTGCAAGTGAAACCTCTTTCATGGA
GTTCTGTCTCATGGCTGTTGCCTTGCTCAGTGTGACCTTGTCGTGTTCTGAACGAGAGCT
ACGGCTGCCTGCTCCAGACCGGTCATGATTGATCATACTCATTGCTGATCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 29-K018512-022-255-B08C-8409
GenBank Accession LN499086 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K018512-022-255-B08C-8409
TGAAGCCATTTACAATTGATCAGCAATGAGTATGATCAATCATGACCGGTCTGGAGCAGG
CAGCCGTAGCTCTCGTTCAGAACACGACAAGGTCACACTGAGCAAGGCAACAGCCATGAG
ACAGAACTCCATGAAAGAGGTTTCACTTGCAAGTGAGATGGAAGTTAACTTCAACGACTA
CTCTCATCGTAACAGTGGAGTATCTAAAGATCAGCAGCAAAGAGCTAAGAAGAGCGGATT
CGCGAGCATTGTGAAGAAAAGCTTCAAAGATCTGACAAAATCTATTCAGAATGATGAAGG
GAACAAAAGCAATGTTTCGATAAATGGACATCCTTTAACCGAACGTCTGCTGAGAAAAGC
AGAGAAGCAAGCCGGAGTCATTCAGCCAGGAAACTACTGGTAAGTAACTTTCGATGCCTC
TTCTGTTATGACATCTTCTTCATGACTGCTAATGTGTTTTGGGGACATTCTAGGTATGAT
TACAGAGCTGGATTCTGGGGAGTAATGGGAGGTCCAGGTCTTGGAATATTACCGGTAAAC
ACATTTTTGATATTGCTTCTTGT

Last Updated on 06.12.2018 16:11