Sequences from confirmation amplicon


Confirmation sequences for line 255F10 and hit At5g18210 are shown.
Line ID 255F10
Locus At5g18210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6018120
Primers used for PCR 05iv - 8474, show primer details
   
Sequence Name 73-K113020-0022-255-F10C-05iv
GenBank Accession LN512930 [GenBank]
Sequencing primer gene specific primer, 05iv: 5'-GAGAAGTCCAAAATCTTAAACCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113020-0022-255-F10C-05iv
CACACTAACGCCGACTTGTTCAGTTCCACAGTCAAAACTCAAAACAGAGTACAGTCACAC
AGTCAACGAAGATACTAATAAGTCAATTTTCGTTTGACCAAATTTGATTCTTCATGGCTT
CCTCAGTCTCCTCTCTCGCCGGACGAGTCGCGATAGTCACCGGTTCTTCTCGCGGAATCG
GCCGAGCTATAGCCATACATCTCGCCGAGCTCGGCGCCAAGATAGTCATCAACTACACCA
CCAGATCCACCGAGGCCGATCAAGTCGCCGCAGAAATCAACTCATCCGCCGGCACCGTTC
CGCAACCGATCGCCGTCGTGTTCCTCGCCGATATCTCAGAACCAAGCCAAATTAAGTCTC
TCTTCGACGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTC
   
Sequence Name 41-K113019-0022-255-F10C-8409
GenBank Accession LN504143 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113019-0022-255-F10C-8409
TTACGTCGAGAGAGACTTAATTTGGCTTGGTTCTGAGATATCGGCGAGGAACACGACGGC
GATCGGTTGCGGAACGGTGCCGGCGGATGAGTTGATTTCTGCGGCGACTTGATCGGCCTC
GGTGGATCTGGTGGTGTAGTTGATGACTATCTTGGCGCCGAGCTCGGCGAGATGTATGGC
TATAGCTCGGCCGATTCCGCGAGAAGAACCGGTGACTATCGCGACTCGTCCGGCGAGAGA
GGAGACTGAGGAAGCCATGAAGAATCAAATTTGGTCAAACGAAAATTGACTTATTAGTAT
CTTCGTTGACTGTGTGACTGTACTCTGTTTTGAGTTTTGACTGTGGAACTGAACAAGTCG
GCGTTAGTGTTGCTAAACGACGGCGTTAGTGTTGCTAAGCGACGCCGTTCGGGGTTTAAG
ATTTGGAACTTCT

Last Updated on 06.12.2018 16:11