Sequences from confirmation amplicon


Confirmation sequences for line 255G01 and hit At3g59820 are shown.
Line ID 255G01
Locus At3g59820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22099230
Primers used for PCR 21dh - 8474, show primer details
   
Sequence Name 21-K109936-0022-255-G01C-21dh
GenBank Accession LN495702 [GenBank]
Sequencing primer gene specific primer, 21dh: 5'-GGTTTTGTATCAGACCATCAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K109936-0022-255-G01C-21dh
CCTCTTAACAATCTCATGGGACTTTAATAGTCTTAAAAACCGATTTAAAATCTTCTTACC
TTCTCCATTAGTGCAGAGGAAACCTCGTCTGCTTCAGCAACTTCATCAGCTTGGTCAATA
TCTACTCTAGCTGCTTTGTATGCCTTCATTGCAGCTTTTTCACCATCTACATCTTCTCTC
TCGACCATTGTGTTATAGAACTCCACCTGGAGAAACAGAAAAACAAAGTCTTTCAATCTA
CGACACAAAGTGCTGATCAAGCATTGGTGGACAGCGTACCTCTTTCTTGACCAATCTCAG
GAATTCTTCGCGCTCTCTACACACCGACTGTTTATATCAAAAGCAAACAGAGTATCAAAT
AAATATCCAATAAACTGCTCTGCATACCCATTGTTGATAACCATAGACTCATTTTTCTTA
CAGAAGCGGAAGCCAAAACGCCCAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
GAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 05-K109935-0022-255-G01C-8409
GenBank Accession LN488399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K109935-0022-255-G01C-8409
CATTTACAATTGAATATATCCTTGGGCGTTTTGGCTTCCGCTTCTGTAAGAAAAATGAGT
CTATGGTTATCAACAATGGGTATGCAGAGCAGTTTATTGGATATTTATTTGATACTCTGT
TTGCTTTTGATATAAACAGTCGGTGTGTAGAGAGCGCGAAGAATTCCTGAGATTGGTCAA
GAAAGAGGTACGCTGTCCACCAATGCTTGATCAGCACTTTGTGTCGTAGATTGAAAGACT
TTGTTTTTCTGTTTCTCCAGGTGGAGTTCTATAACACAATGGTCGAGAGAGAAGATGTAG
ATGGTGAAAAAGCTGCAATGAAGGCATACAAAGCAGCTAGAGTAGATATTGACCAAGCTG
ATGAAGTTGCTGAAGCAGACGAGGTTTCCTCTGCACTAATGGAGAAGGTAAGAAGATTTT
AAATCGGTTTTTAAGACTATTAAAGTCCCATGAGATTTGTTTAAGAGGCTTACTCTTCGG
TCATACTTTTTAAGGTTGATGGTCTGATACAAAA

Last Updated on 06.12.2018 16:11