Sequences from confirmation amplicon


Confirmation sequences for line 255G07 and hit At2g39980 are shown.
Line ID 255G07
Locus At2g39980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16689693
Primers used for PCR MZ32 - 8474, show primer details
   
Sequence Name 39-K023834-022-255-G07C-8409
GenBank Accession LN503216 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K023834-022-255-G07C-8409
AGATTTCCCGGAACAATGAAGCCATCTTCTTCAAAAGTAAACCCATCTCTCTCAATCACT
CTCACCCACTTCCCTCCTCTCGCTGGCCGTCTCTCAACCTCCTCCTCCGGCCACGTTTTC
CTCACCTGCAATGACGCCGGCGCCGATTTTGTTTTCGCCCAAGCAAAATCCATCCACGTG
AGCGACGTGATCGCCGGAATCGACGTTCCCGATGTAGTCAAAGAGTTTTTCACCTACGAC
AGAGCTGTGAGCTACGAGGGACATAACAGACCAATCCTCGCCGTTCAAGTGACGGAACTG
AACGACGGCGTTTTCATCGGATGCTCCGTTAACCACGCCGTGACTGACGGAACCTCGCTG
TGGAATTTCATTAACACCTTCGCGGAGGTTTCTAGAGGTGCTAAGAATGTGACACGTCAG
CCTGATTTTACGCGGGAGTCTGTGCTAATCTCACCGGCTGTGCTCAAAGTCCCTCAAGGT
GGTCCAAAGGTGACATTTGACGAAAACGCGCCGTTACGTGAACGGATATTCAGTTTCAGT
AGAGAATCGATTCAAGAGCTTAAAGCTGTGGT
   
Sequence Name 43-K023834-022-255-G07C-MZ32
GenBank Accession LN504704 [GenBank]
Sequencing primer gene specific primer, MZ32: 5'-TTCTTCTTGTTCACCACAGCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K023834-022-255-G07C-MZ32
CTCTACTGAAACTGAATATCCGTTCACGTAACGGCGCGTTTTCGTCAAATGTCACCTTTG
GACCACCTTGAGGGACTTTGAGCACAGCCGGTGAGATTAGCACAGACTCCCGCGTAAAAT
CAGGCTGACGTGTCACATTCTTAGCACCTCTAGAAACCTCCGCGAAGGTGTTAATGAAAT
TCCACAGCGAGGTTCCGTCAGTCACGGCGTGGTTAACGGAGCATCCGATGAAAACGCCGT
CGTTCAGTTCCGTCACTTGAACGGCGAGGATTGGTCTGTTATGTCCCTCGTAGCTCACAG
CTCTGTCGTAGGTGAAAAACTCTTTGACTACATCGGGAACGTCGATTCCGGCGATCACGT
CGCTCACGTGGATGGATTTTGCTTGGGCGAAAACAAAATCGGCGCCGGCGTCATTGCAGG
TGAGGAAAACGTGGCCGGAGGAGGAGGTTGAGAGACGGCCAGCGAGAGGAGGGAAGTGGG
TGAGAGTGATTGAGAGAGATGGGTTTACTTTTGAAGAGATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11