Sequences from confirmation amplicon


Confirmation sequences for line 256B08 and hit At4g21630 are shown.
Line ID 256B08
Locus At4g21630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11494180
Primers used for PCR HN26 - 8474, show primer details
   
Sequence Name 51-K118152-0022-256-B08C-HN26
GenBank Accession LN507633 [GenBank]
Sequencing primer gene specific primer, HN26: 5'-TCCAAATCCAACTTCTTTTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118152-0022-256-B08C-HN26
CAACATGGGCAGTGAAGCTATCATCGGCGTCGTTGATACCGGTCTTTCTCTCGCTCTCTA
AATATAACTCTATCTCTATCTTTCTCCTTGATTCACATCATGTATGTATGTATATCTATG
CAGGAATATGGCCGGAGTCAAAGGTCTTCAACGATCATGGTCTTGGACCAATACCTCAGC
GTTGGAGAGGAAAATGTGAATCAGGAGAACAGTTTAACGCCAAAATACATTGCAACAACA
AGCTAATAGGAGCAAAGTACTATCTAAGCGGTCTACTCGCCGAGACTGGAGGGAAATTCA
ACAGAACAATCATCCAAGATTTCAAATCCAACAGAGATGCAATTGGTCACGGCACGCATA
CAGCCACAATAGCCGGTGGCTCATTCGTTCCCAACGTGAGCTTCTATGGCCTAGCCCGAG
GTACTGATATAGCAATGAGTATTAGCAGCAACAGTCAAGAGCCAAGTGTGTTTTTTTAAG
TGTGATCGGTGTTAGCATACTCGCGGCAGTGGTTATCAGGCTACAGAATAAAGCTTTTCT
GTTAACCACAAGAGCAATGAGTATTGACCGGTGACTGTTGCTGCTAATTTTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCNAGCGT
TATTAT
   
Sequence Name 03-K118151-0022-256-B08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118151-0022-256-B08C-8409
TACATTCTATGCTTGTATCTTTGCCGCTCGCAATAGCATATAA

Last Updated on 06.12.2018 16:11