Sequences from confirmation amplicon


Confirmation sequences for line 256C12 and hit At2g39330 are shown.
Line ID 256C12
Locus At2g39330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16420245
Primers used for PCR 21fb - 8474, show primer details
   
Sequence Name 19-K111392-0022-256-C12C-21fb
GenBank Accession LN494815 [GenBank]
Sequencing primer gene specific primer, 21fb: 5'-TTAGAGTTCGAGTACGAGAAAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K111392-0022-256-C12C-21fb
CAGAAGATCACTGAAGGTAAATTTCTAGAAACTGTGAGATATAGTAGAACAACCAAAATT
TAGGTCTTAAATATAACTTTTGATATCTTGCGACAGAAGTATTCTTAAATTTTGATTTTG
TTGCAGTTTGAGCTTAACCCGGATGAATACATCAAATCGGTGGAAGCAACCTATGATAAA
CCGGACATTTTCCGCAATGTCGTCATTACATCGCTTACATTTGAAACATCGAAGGGGAGA
ACGTCATTCTCTGGGTATAAGGGAGGTAAGAAGTTTAAGCTAGAGCAAAAGGGTCGTAGG
CTTGTCGGGTTCCATGGAAAGGAAGGTTCAGCTATTGATGCCCTTGGAGCATATTTTGCA
CCTATTCCTACTCCGACTCCTATAATTCCAGCCAAGAAACTACCAGCAATAGGCGGCAGC
GGAGGAGTTGCATGGGATGATGGTGTCTACGATGGTGTAAGGAAGATACTTGTGTCCATT
TACATGGATCAGCAATGAGTATGATGGCTGATCCATCGTTTCCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGG
TTATTA
   
Sequence Name 03-K111391-0022-256-C12C-8409
GenBank Accession LN487545 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K111391-0022-256-C12C-8409
CATTTACATTGAATTATCCTGGAAACGATGGATCAGCCATCATACTCATCGCTGATCCAT
GTAAATGGACACAAGTATCTTCCTTACACCATCGTAGACACCATCATCCCATGCAACTCC
TCCGCTGCCGCCTATTGCTGGTAGTTTCTTGGCTGGAATTATAGGAGTCGGAGTAGGAAT
AGGTGCAAAATATGCTCCAAGGGCATCAATAGCTGAACCTTCCTTTCCATGGAACCCGAC
AAGCCTACGACCCTTTTGCTCTAGCTTAAACTTCTTACCTCCCTTATACCCAGAGAATGA
CGTTCTCCCCTTCGATGTTTCAAATGTAAGCGATGTAATGACGACATTGCGGAAAATGTC
CGGTTTATCATAGGTTGCTTCCACCGATTTGATGTATTCATCCGGGTTAAGCTCAAACTG
CAACAAAATCAAAATTTAAGAATACTTCTGTCGCAAGATATCAAAAGTTATATTTAAGAC
CTAAATTTTGGTTGTTCTACTATATCTCACAGTTTCTAGAAATTTACCTTCAGTGATCTT
TCTTGTTTCACCCCATGGCGACATGTCTCTAACTTACCGCCTTTCTCGTACTCGAACTCT

Last Updated on 06.12.2018 16:11