Sequences from confirmation amplicon


Confirmation sequences for line 256F06 and hit At3g21220 are shown.
Line ID 256F06
Locus At3g21220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7446427
Primers used for PCR 39qn - 8474, show primer details
   
Sequence Name 87-K115691-0022-256-F06C-39qn
GenBank Accession LN514794 [GenBank]
Sequencing primer gene specific primer, 39qn: 5'-TTCTCATTATCTTTGCTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K115691-0022-256-F06C-39qn
AATTGAAAAAAAGAAAGACGAAAATGGTAGAAACACTAAGAGGCAGAAGGAAGAGGACGA
GGCGGCGGCAACATTTGACGGAGATTAGGACCACCGGTAGCTTTAAGTATGAAAGGATGC
TGCAAAAGCTGTTGAGCTGACCATCTCTTAGGAGGATCACTCTGTAAACAACAAGAAACA
AAGTGACGAAACTCCTGAGACGCCGTAGCCGGAGCTTCAGGTGGCTGAGACATACAAATA
GCACACATAAGACTAGCCCAATCACCTTGTCTACTCACAGCAAAAGGAAACCTCCCCAAG
TAAAACTCCAAGATACTAACACCTAAACTCCAAACATCTCCAGCATAACCATCGTAACGA
CCATGATTCAAATCAGTATTAATCCTCTCAGGACTCATATAAGCAATAGTACCAACAGAT
GAATTACAAGGATCCATTGTTTGTGCCAAGATCCTACTCACACCAAAATCAGCAATTTTC
ACATTTTTAGCTGAGTTTATAAGAAGATTCGAAGGTTTGATATCACGATGAACGATATGA
CGACGATGAAGATAAGCTAATCCACTAAGAATCTGACGAGAGAGATCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 39-K115690-0022-256-F06C-8409
GenBank Accession LN503471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115690-0022-256-F06C-8409
ATTCTTANTGGATTAGCTTATCTTCATCGTCGTCATATCGTTCATCGTGATATCAAACCT
TCGAATCTTCTTATAAACTCAGCTAAAAATGTGAAAATTGCTGATTTTGGTGTGAGTAGG
ATCTTGGCACAAACAATGGATCCTTGTAATTCATCTGTTGGTACTATTGCTTATATGAGT
CCTGAGAGGATTAATACTGATTTGAATCATGGTCGTTACGATGGTTATGCTGGAGATGTT
TGGAGTTTAGGTGTTAGTATCTTGGAGTTTTACTTGGGGAGGTTTCCTTTTGCTGTGAGT
AGACAAGGTGATTGGGCTAGTCTTATGTGTGCTATTTGTATGTCTCAGCCACCTGAAGCT
CCGGCTACGGCGTCTCAGGAGTTTCGTCACTTTGTTTCTTGTTGTTTACAGAGTGATCCT
CCTAAGAGATGGTCAGCTCAACAGCTTTTGCAGCATCCTTTCATACTTAAAGCTACCGGT
GGTCCTAATCTCCGTCAAATGTTGCCGCCGCCTCGTCCTCTTCCTTCTGCCTCTTAGTGT
TTCTACCATTTTCGTCTTTCTTGTTTTTCAATTTTTCGTAGTTTTTCCTGACATTTTTCC
ATAAGTTTTGCATACCCCAAGCAAAATNAATGAG

Last Updated on 06.12.2018 16:11