Sequences from confirmation amplicon


Confirmation sequences for line 256H06 and hit At5g48370 are shown.
Line ID 256H06
Locus At5g48370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19598936
Primers used for PCR 17uw - 8474, show primer details
   
Sequence Name 60-K118051-0022-256-H06C-17uw
GenBank Accession LN510364 [GenBank]
Sequencing primer gene specific primer, 17uw: 5'-TAATGCATAGAGCATTTGAGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118051-0022-256-H06C-17uw
TTGATCCACGTCGATTTTCTAAGACCGGTTCGTAACCAAATCACTTCTTACCATTATCTT
CTTGTTCACGTTCGTCTTCTGTGTAAACTTACGCTTAATGGACTTAAATGACAGGTGGAC
GTCGGGGATTTCTTACGTTTCAAATCCTGTGTTCTTTACACTCAACTGGATAAACAGGAT
TGTCCGCTCATCAATATCGAAGTTGTTGCTCATGTTACAAGTCCAGAGATTCGCTCCAGT
GAGGTAAAATTCTAACTTGATGATGGAGCTTTCTTAAACGGTTGTTAAAATTGAACTTGC
TCACTGATTCTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCG
   
Sequence Name 24-K118050-0022-256-H06C-8409
GenBank Accession LN497175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118050-0022-256-H06C-8409
TAGAATCAGTGAGCAAGTTCAATTTTAACAACCGTTTAAGAAAGCTCCATCATCAAGTTA
GAATTTTACCTCACTGGAGCGAATCTCTGGACTTGTAACATGAGCAACAACTTCGATATT
GATGAGCGGACAATCCTGTTTATCCAGTTGAGTGTAAAGAACACAGGATTTGAAACGTAA
GAAATCCCCGACGTCCACCTGTCATTTAAGTCCATTAAGCGTAAGTTTACACAGAAGACG
AACGTGAACAAGAAGATAATGGTAAGAAGTGATTTGGTTACAAACCGGTCTTAGGAAATC
GACGTGATCAACTTCTAAGAAGTAAGGCACTAGACCCGCAAACGTATAAGCAGTAGAGAA
AGCCAACTCAAATGCTCAAATGCATTA

Last Updated on 06.12.2018 16:11