Sequences from confirmation amplicon


Confirmation sequences for line 257A11 and hit At5g11050 are shown.
Line ID 257A11
Locus At5g11050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3503323
Primers used for PCR RS04 - 8474, show primer details
   
Sequence Name 43-K017194-022-257-A11C-RS04
GenBank Accession LN504663 [GenBank]
Sequencing primer gene specific primer, RS04: 5'-GTAGCTAGAACAATGGGAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017194-022-257-A11C-rs04
AACATTTCTATCAGATCCATTTCTCTTCTTTCGCTAGCGGAGTTAGCTTGGGGCAAAAGC
AACTCGATTTCTCCGGCATGCTCATTGTTGTTGGAAGACAGAAATGTAAGTGAAGAAGAA
GAGGACGCCGTACCGTTCAACAAATCAGATAAGTAGATATCAGATGCGAGGTGGGAAGAA
TTGGCAGGTTCTGTGACCATTGCTCCAAGATGTGTTCCAAAAGTATTGTTGTACAAGTTA
GGTTAAGAGTTTGGGTTCTCGATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAT
   
Sequence Name 47-K017194-022-257-A11C-8409
GenBank Accession LN506032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K017194-022-257-A11C-8409
AAGCCATTTCCAATTGAATATGATCGAGAACCCTAAACCTAAACCTAACTTGGACAACAA
TACTTTTGGAACACATCTTGGAGCAATGGTCACAGAACCTGCCAATTCTTCCCACCTCGC
ATCTGATATCTACTTATCTGATTTGTTGAACGGTACGGCGTCGTCTTCTTCTTCACTTAC
TTTTCTGTCTTCCAACAACAATGAGCATGCCGGAGAAAAACGAGTTGCTTTTGCCCCAAG
CTAACTCCACTAGCGAAAGAAGAGAAATGGATCTGATAGAAATGTTGTCTGGTTCTACTC
AAGGTAGCAACATTTGGTTCCCATTGTTCTAGCTA

Last Updated on 06.12.2018 16:11