Sequences from confirmation amplicon


Confirmation sequences for line 257G02 and hit At4g23550 are shown.
Line ID 257G02
Locus At4g23550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12291809
Primers used for PCR IS19 - 8474, show primer details
   
Sequence Name 23-K017255-022-257-G02C-IS19
GenBank Accession LN496350 [GenBank]
Sequencing primer gene specific primer, IS19: 5'-AAATTTCGCATTTTAAAGAACCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017255-022-257-G02C-IS19
CTGACCTTTTCTTTGATTTGGATTCTGAAATTCGGATATGATCAGCTCTTGATCCGTTGG
ATAAAAGACAACCATGTGATCGTTGTTTCTTGTCATCTCGGAAAGGTTTTGAATCTTCAG
GGAGAGAGACCGAGCTTGTGAGGATCGTTTGTGTGGAGAAAGGGTAAAAGGGTTTGTAAA
GTTCACCGAGCTCATCTAAGCCACTTGTCTCCATCTCCGGTTCGTAGAAACTAGACGTCT
CCATCGGTAGGCAAAACGAAGGTGAAAACGTACCGGAACTTTCGCCGGAAAACGCGTCTC
CGGAGCCGGAGTAGCCTCTGACTATTGCTTCTAGGTCTCCTTCGTCCATATAAGCCACCT
CACCCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCA
   
Sequence Name 24-K017255-022-257-G02C-8409
GenBank Accession LN496792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017255-022-257-G02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATGGGTGAGGTGGCTTATATGG
ACGAAGGAGACCTAGAAGCAATAGTCAGAGGCTACTCCGGCTCCGGAGACGCGTTTTCCG
GCGAAAGTTCCGGTACGTTTTCACCTTCGTTTTGCCTACCGATGGAGACGTCTAGTTTCT
ACGAACCGGAGATGGAGACAAGTGGCTTAGATGAGCTCGGTGAACTTTACAAACCCTTTT
ACCCTTTCTCCACACAAACGATCCTCACAAGCTCGGTCTCTCTCCCTGAAGATTCAAAAC
CTTTCCGAGATGACAAGAAACAACGATCACATGGTTGTCTTTTATCCAACGGATCAAGAG
CTGATCATATCCGAATTTCAGAATCCAAATCAAAGAAAAGGTCAGTATTCTTGAAGTTCT
TTAAGAGGGTTCTTTAAAATCGGAAATT

Last Updated on 06.12.2018 16:11