Sequences from confirmation amplicon


Confirmation sequences for line 259A06 and hit At1g61320 are shown.
Line ID 259A06
Locus At1g61320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22619118
Primers used for PCR 21cy - 8474, show primer details
   
Sequence Name 12-K109236-0022-259-A06C-21cy
GenBank Accession LN491619 [GenBank]
Sequencing primer gene specific primer, 21cy: 5'-CAGATGTTGATGTTCATTTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K109236-0022-259-A06C-21cy
AGATTCTTAGACTTACATGGTATAAGAATCCAATACATTCTCAAAAGGTATAAATCACAT
CAATATATATATATATATAGGACTTAGCAAGAATTATAATCTTATACCAACATAACAGTG
CATCCAAATATTATTTGTTTGACTAGGTATTTGATCGAGAAATTTCACTAAATCTTATAC
CTTAAAACTTAAGATACTTTTTTTTGTTACAAGCATTATTCTTAAAGTGGTTGGATTTTA
GAGTTTGCACAAACTCTCTTGATGATCATGATAGAAGATGATATCTCATCGATATCATAT
ATAACGTATGGAATTTTCACATGATTTTGTCACCTTATGGTTGTCTCCATTTTCTTTTGT
TCATAATCTTTCATTCTTGATTTAGAGCGAGTTTTGATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCTTTATT
   
Sequence Name 04-K109235-0022-259-A06C-8409
GenBank Accession LN487957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109235-0022-259-A06C-8409
GCCATTTACATCAAACTCGCTCTAATCAAGAATGAAAGATTATGAACAAAAGAAAATGGA
GACAACCATAAGGTGACAAAATCATGTGAAAATTCCATACGTTATATATGATATCGATGA
GATATCATCTTCTATCATGATCATCAAGAGAGTTTGTGCAAACTCTAAAATCCAACCACT
TTAAGAATAATGCTTGTAACAAAAAAAAGTATCTTAAGTTTTAAGGTATAAGATTTAGTG
AAATTTCTCGATCAAATACCTAGTCAAACAAATAATATTTGGATGCACTGTTATGTTGGT
ATAAGATTATAATTCTTGCTAAGTCCTATATATATATATATATTGATGTGATTTATACCT
TTTGAGAATGTATTGGATTCTTATACCATGTAAGTCTAAGAATCTTATCTAAATATCAAT
TAGTAAGAAAAAAAAGTAATACAAATACAAACAAATGAACATCCACCTCTG

Last Updated on 06.12.2018 16:11