Sequences from confirmation amplicon


Confirmation sequences for line 259A07 and hit At3g19850 are shown.
Line ID 259A07
Locus At3g19850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6898591
Primers used for PCR 23cy - 8474, show primer details
   
Sequence Name 21-K109134-0022-259-A07C-23cy
GenBank Accession LN495695 [GenBank]
Sequencing primer gene specific primer, 23cy: 5'-ATGTGCGTCGTATAACAGAAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K109134-0022-259-A07C-23cy
AATGCCTCAATCTAGAGCAATATAGAACGTATTTGGAAGTGTATAATCCATGATTACTCA
AATTACAAAATATCCATTTTATTTGCATCCACAAGGACATAATTATATGCATTCTCAAAA
CTACGTAAAATACATTTTGCATGAAAAAGCTTATAATATACACGAGACGTGCATGTGTGT
GATGATGATATGATGACATAAAAATCTATATCTAGAGAAATAAGAAAAAAATGACGAGTG
AATTAATTATTTAACAAAGCCTTGGAAAACTAGAACTTGAATGTGTATGTCCATGTCTCA
CAGATCTTGACATTACTTCACTCTTCTTCTCCTTACTAACTTTTTCAGAATTATTCCATT
GCTTCCTCTCCAAATTCATCATTAGATCATCTTCAAACTTCTCCGACATATCAAAACCTT
TCAAACACACCAGTATTGGTTTCTCTTCTAAGTACTTACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K109133-0022-259-A07C-8409
GenBank Accession LN488391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K109133-0022-259-A07C-8409
CTTTACATTGAATATATCCTGTAAGTACTTAGAAGAGAAACCAATACTGGTGTGTTTGAA
AGGTTTTGATATGTCGGAGAAGTTTGAAGATGATCTAATGATGAATTTGGAGAGGAAGCA
ATGGAATAATTCTGAAAAAGTTAGTAAGGAGAAGAAGAGTGAAGTAATGTCAAGATCTGT
GAGACATGGACATACACATTCAAGTTCTAGTTTTCCAAGGCTTTGTTAAATAATTAATTC
ACTCGTCATTTTTTTCTTATTTCTCTAGATATAGATTTTTATGTCATCATATCATCATCA
CACACATGCACGTCTCGTGTATATTATAAGCTTTTTCATGCAAAATGTATTTTACGTAGT
TTTGAGAATGCATATAATTATGTCCTTGTGGATGCAAATAAAATGGATATTTTGTAATTT
GAGTAATCATGGATTATACACTTCCAAATACGTTCTATATTTGCTCTTAGATTTGAGGCA
TTTGCTCAGAGGATGTAGAAAACAAAAAATAATCAGTTTCTGTTATACG

Last Updated on 06.12.2018 16:11