Sequences from confirmation amplicon


Confirmation sequences for line 259D05 and hit At2g17470 are shown.
Line ID 259D05
Locus At2g17470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7586136
Primers used for PCR CL77 - 8474, show primer details
   
Sequence Name 49-K032927-022-259-D05C-8409
GenBank Accession LN506740 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K032927-022-259-D05C-8409
CCATTTCATATATATACACATCTATAATATGATTTTACATTTTACGAATTGGATTGGTCC
ATCATAAAAAAATTCCATCTTTGTTTTCAAGGACCATTGAAGTAATATTTGTTTACCTTC
TAGGGAATTTGCCACACTCTTGAAATTATTGGCCACCAGTTTATGGAGATCCTCCCCGGC
CCATATTGGAAATATGAATATATTCACAACCAAACATGTGGTTGCACCAACAACAATAAA
CAAAAACCTGTAATACGCCGTGCTGAAGAAGTCTCCGGTATTGTTCCCCGAAACCAGAAC
AATACAGAATGTCAACAAGAACACCCTGAATGCGTATTCATATGGCTTCATGGCCGGGTG
GAGTTTTGAATAACTAGCAATGAAACCTGGTAGGTCAAGTTCAAAGCGAAAATGTTACTA
TTTATCTTCAAAACCTCATGTAGAAGACAAGAAGACAGGGGAAATGCA
   
Sequence Name 49-K032927-022-259-D05C-CL77
GenBank Accession LN506741 [GenBank]
Sequencing primer gene specific primer, CL77: 5'-GCATTTCCTTGTCTTCTTGTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K032927-022-259-D05C-CL77
GTACATTTTCGCTTTGACTTGACCTACCAGGTTTCATTGCTAGTTATTCAAAACTCCACC
CGGCCATGAAGCCATATGAATACGCATTCAGGGTGTTCTTGTTGACATTCTGTATTGTTC
TGGTTTCGGGGAACAATACCGGAGACTTCTTCAGCACGGCGTATTACAGGTTTTTGTTTA
TTGTTGTTGGTGCAACCACATGTTTGGTTGTGAATATATTCATATTTCCAATATGGGCCG
GGGAGGATCTCCATAAACTGGTGGCCAATAATTTCAAGAGTGTGGCAAATTCCCTAGAAG
GTAAACAAATATTACTTCAATGGTCCTTGAAAACAAAGATGGAATTTTTTTATGATGGAC
CAATCCAATTCGTAAAATGTAAAATCATATTATAGATGTGTATATATATGAAATGGCTTA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11