Sequences from confirmation amplicon


Confirmation sequences for line 259F06 and hit At5g24440 are shown.
Line ID 259F06
Locus At5g24440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8346405
Primers used for PCR 17xc - 8474, show primer details
   
Sequence Name 37-K119070-0022-259-F06C-17xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 17xc: 5'-CTTTCTTGTGGCCAGGTACTTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119070-0022-259-F06C-17xc
CAACTCTTTCTCTAAGTTTTCTTGCCATTTTTTTTTGGGTAAAGGTTGTTGATTGCCGTA
TGTGCGGTGACTATAAGTCCATTCTTCGTTTCGCCTTCATTGAGTTCACCGATGCAGGTA
CTCAATTATGCGCCAATTTTCTTATGAGTTGAGATGTTCTTGCTGTTTTTTTATTAATCA
GTGAAGTGATGTCTCAAAGGGAGCTAGGTCTGCTTTACGAAAATCCGGTACTATGTTTGG
TTCTCATCCTATTAGGGTTTTTATGTCCAAAACAGCTATTGCGCCTGTTAACCCGTCTTT
CCTTCCACAAGTAAGTTGAGATAATCATGTCTAAACCAATCTTAGCTAGGCTTGACTAGA
TAATTTTGAATCAATCTTGTTCTTATCAATAATGTGGTATATGCATAACGTTGTTTTCTT
CCTTCAGTCCAAGGACGAGCTAGAGAAGTGTGGAAAGACTGTTTATTGTACCAATTTAAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 05-K119069-0022-259-F06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119069-0022-259-F06C-8409
TCGGATGAGCCATTTCATTTAAATTGGTACAATAAACAGTCTTTCCACACTTCTCTAGCT
CGTCCTTGGACTGAAGGAAGAAAACAACGTTATGCATATACCACATTATTGATAAGAACA
AGATTGATTCAAAATTATCTAGTCAAGCCTAGCTAAGATTGGTTTAGACATGATTATCTC
AACTTACTTGTGGAAGGAAAGACGGGTTAACAGGCGCAATAGCTGTTTTGGACATAAAAA
CCCTAATAGGATGAGAACCAAACATAGTACCGGATTTTCGTAAAGCAGACCTAGCTCCCT
CTGAGACATCACTTCACTGATTAATAAAAAAACAGCAAGAACATCTCAACTCATAAGAAA
ATTGGCGCATAATTGAGTACCTGCATCGGTGAACTCAATGAAGGCGAAACGAAGAATGGA
CTTATAGTCACCGCACATACGGCAATCAACAACCTTTAACCCAAAAAAAAATGGCAAGAA
AACTTATGAGAAATGAGTTTGCATAAAATCAAAACGAAGATCATTAAGTAAGTACCTGGC
CACAAGAAA

Last Updated on 06.12.2018 16:11