Sequences from confirmation amplicon


Confirmation sequences for line 259H08 and hit At3g47770 are shown.
Line ID 259H08
Locus At3g47770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17620947
Primers used for PCR 07gg - 8474, show primer details
   
Sequence Name 79-K111813-0022-259-H08C-8409
GenBank Accession LN513844 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K111813-0022-259-H08C-8409
TGAAACTTCATCCATTGCACTACCTCTGAAATCTTTCCACTTCATCCCATCCCTCCCATT
CAAGTTTCTTTGGAAAGCATATTGGGAGAATTCATACAACCCGCGGTATAATGAGAAGCC
GGGATACAATTCCATGACAAAAATCCAACGTCCTGGTAAAAAGTAAAAGATTATATATAG
TAGCATTGTGGTCTGTTTTGAGAATATGTTATCTTCAAGTTGTTATTGATAGCTTACTAG
GAAACGATAAACTTTCTATCAGAAACTGGAAGAGGAATGCGCCCAAGAGCCCAGATCCAA
ACACGTATATGTAAGCAACAACTACAATTTGGGAATAAAACAGAAAGTGTTTCTTTTTAA
CACCAACATGTTCTTGACCAAATTTGAAAAATGTGACTTCATTTAGTACGTACCTGATGC
TGTCACAGCCTTTGAAAATGCTGAGGAAACTAGAAAGGC
   
Sequence Name 87-K111814-0022-259-H08C-07gg
GenBank Accession LN514780 [GenBank]
Sequencing primer gene specific primer, 07gg: 5'-TCTGCAAATATCCATAGCCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K111814-0022-259-H08C-07gg
ACGTACTAATGAAGTCCATTTTTCAATTTGGTCAAGAACATGTTGGTGTTAAAAAGAAAC
ACTTTCTGTTTTATTCCCAAATTGTAGTTGTTGCTTACATATACGTGTTTGGATCTGGGC
TCTTGGGCGCATTCCTCTTCCAGTTTCTGATAGAAAGTTTATCGTTTCCTAGTAAGCTAT
CAATAACAACTTGAAGATAACATATTCTCAAAACAGACCACAATGCTACTATATATAATC
TTTTACTTTTTACCAGGACGTTGGATTTTTGTCATGGAATTGTATCCCGGCTTCTCATTA
TACCGCGGGTTGTATGAATTCTCCCAATATGCTTTCCAAAGAAACTTGAATGGGAGGGAT
GGGATGAAGTGGAAAGATTTCAGAGGTAGTGCAATGGATGAAGTTTCATTAACGATAATG
ATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCA

Last Updated on 06.12.2018 16:11