Sequences from confirmation amplicon


Confirmation sequences for line 260E03 and hit At5g49970 are shown.
Line ID 260E03
Locus At5g49970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20330235
Primers used for PCR 33jx - 8474, show primer details
   
Sequence Name 73-K113379-0022-260-E03C-33jx
GenBank Accession LN512935 [GenBank]
Sequencing primer gene specific primer, 33jx: 5'-AATTGCTGAGGTAATTCGATCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113379-0022-260-E03C-33jx
TATCTGAATGTAAAGCTCAAAGCTTTAACCTTTGAACCGATAAACCTCTAAAGCTCTCTC
TTTTCCTTGGATGAGTCTCACAAGTTAAGAACTTCAGTGAAATAATCTGACTTTATTGAA
CCCAAACTTGGGTATCACTGTTTATCTTAGCATTACAGAGTTTTGTTTTTGTTATGTACA
TTGGATTTGAAGTCTACAATGTTTTTCCAGGTTTATAAACCGGAAGAATATAGCCGGGTT
CTAGCTATCTGTGGTCCTGGGAACAATGGTGGTGATGGTTTGGTGGCGGCGAGGCATTTG
CACCACTTTGGATATAAACCGTTTATTTGTTATCCCAAACGTACAGCCAAGCCACTTTAT
ACTGGACTGGTCACTCAGGTTTGTGTAACCAGTGCTTAATTTATGGGGGATCTTTGTTAG
CTTTCTCCGTTTCTTTACTGCCTGCTGAATTTGCCTGTTTTTGTAGTTGGATTCACTCTC
AGTCCCTTTTGTTTCCGTTGAGGATCTGCCGGATGACTTGTCAAAGGACTTTGATGTTAT
TGTAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 35-K113377-0022-260-E03C-8409
GenBank Accession LN501901 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K113377-0022-260-E03C-8409
ATAACATCAAAGTCCTTTGACAGTCATCCGGCAGATCCTCAACGGAAACAAAAGGGACTG
AGAGTGAATCCAACTACAAAAACAGGCAAATTCAGCAGGCAGTAAAGAAACGGAGAAAGC
TAACAAAGATCCCCCATAAATTAAGCACTGGTTACACAAACCTGAGTGACCAGTCCAGTA
TAAAGTGGCTTGGCTGTACGTTTGGGATAACAAATAAACGGTTTATATCCAAAGTGGTGC
AAATGCCTCGCCGCCACCAAACCATCACCACCATTGTTCCCAGGACCACAGATAGCTAGA
ACCCGGCTATATTCTTCCGGTTTATAAACCTGGAAAAACATTGTAGACTTCAAATCCAAT
GTACATAACAAAAACAAAACTCTGTAATGCTAAGATAAACAGTGATACCCAAGTTTGGGT
TCAATAAAGTCAGATTATTTCACTGAAGTTCTTAACTTGTGAGACTCATCCAAGGAAAAG
AGAGAGCTTTAGAGGTTTATCGGTTCAAAGGTTAAAGCTTTGAGCTTTACATTCAGATAC
CTACAATGAAACGTCTCACACATATGTCTAGATCTAAAGTGATCGAAT

Last Updated on 06.12.2018 16:11