Sequences from confirmation amplicon


Confirmation sequences for line 261H03 and hit At2g24480 are shown.
Line ID 261H03
Locus At2g24480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10398065
Primers used for PCR 09vz - 8474, show primer details
   
Sequence Name 60-K118278-0022-261-H03C-09vz
GenBank Accession LN510367 [GenBank]
Sequencing primer gene specific primer, 09vz: 5'-TCTGAAACTTTGGTAGTGTCATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118278-0022-261-H03C-09vz
TTTGGTAAGCCACTTCAAAGTTCAAGTTGATTTTTTGTTTAATCTGAAACTATATTTGGT
TTAAGACAGCGTTTTGTGTTGCAATGTCGCATACTAGAATCAAACTTGTAAATGAAACTG
CAATTACATGTTACCAACAAAAACAGATACATAAACTCTGAAAACCCTAATTTGCCTCTA
GACAAAACAATGAAACATAAAAGTGAAAACCCTAAACACACTTCCGAAACAAGCTAAGTT
TACAAAGTCACTAAAGACCACAAAATAAAACCCTAATTAGACTCTGCTATTTGTCAACAG
GTTTGCGACAGAGAGGGCAAGAGTTATTATGACCAATCACCCACTTGGTGACACATCCTT
CATGATAACAATGCTTACAATAAGGCATCTGGCAATAATTCTCAGAAATTGTGGAATTGT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
T
   
Sequence Name 28-K118277-0022-261-H03C-8409
GenBank Accession LN499040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118277-0022-261-H03C-8409
CACAATTCCACAATTTCTGAGAATTATTGCCAGATGCCTTATTGTAAGCATTGTTATCAT
GAAGGATGTGTCACCAAGTGGGTGATTGGTCATAATAACTCTTGCCCTCTCTGTCGCAAA
CCTGTTGACAAATAGCAGAGTCTAATTAGGGTTTTATTTTGTGGTCTTTAGTGACTTTGT
AAACTTAGCTTGTTTCGGAAGTGTGTTTAGGGTTTTCACTTTTATGTTTCATTGTTTTGT
CTAGAGGCAAATTAGGGTTTTCAGAGTTTATGTATCTGTTTTTGTTGGTAACATGTAATT
GCAGTTTCATTTACAAGTTTGATTCTAGTATGCGACATTGCAACACAAAACGCTGTCTTA
AACCAAATATAGTTTCAGATTAAACAAAAAATCAACTTTGAACTTTTGAAGTTGGCTTAC
CAAATTGAAAGAAACAAGGAAACAATCCAACACCAGAATTAAGCATGACACTACCAAGGT
TTCAGA

Last Updated on 06.12.2018 16:11