Sequences from confirmation amplicon


Confirmation sequences for line 262B03 and hit At1g08730 are shown.
Line ID 262B03
Locus At1g08730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2782555
Primers used for PCR AS28 - 8474, show primer details
   
Sequence Name 29-K023623-022-262-B03C-8409
GenBank Accession LN499120 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K023623-022-262-B03C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACCGAGAAAGGACAAT
GTTAACAAAATAACTCCACATACCGGTTGGATTTTGTTATCTCACTTTATTTCCCCTTTT
ATTTTCCATCTGCAGAAGCCTGGTGGAATTGTTGCTCTCCTCGATGAAGCTTGGTAAAGT
TAAAGATCAAACTAGTTCCATATGAAGCATGAGGTGATTATGTTCTTGATTTGAACTAAC
GAGGTGTAATTTTTCAGCATGTTTCCAAAGTCAACCCACGAAACATTTGCAAATAAGCTG
TATCAGACTTTTAAGACACACAAGCGGTTCATCAAACCAAAACTCTCTCGAACAGATTTC
GCTGTTGCCCATTATGCTGGAGAAGTAGGTTCTCTCTCCTTTGAAGAAAAATGGACCTTG
TAAAACACAGAAGTTTCTCATGTCTTCTAAATCTCCAGGTTCTATATCAGTCTGAACTAT
TTCTGGATAAAAACAAGGATTATGTGATCCCTGAACACCAAGATTTGTTGGGGGCTTCCA
AATGTCCCTTTGTTGTGGGTCTTTTCCCTCCACTCCCAGAAGAAACATCTAAATCGTCAA
AGTTTTCATCCATTGGTTCTCGCTTTAAGGTAGGGAAGCTT
   
Sequence Name 30-K023623-022-262-B03C-AS28
GenBank Accession LN499557 [GenBank]
Sequencing primer gene specific primer, AS28: 5'-TGAACATATGAACAAGCTTCCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K023623-022-262-B03C-AS28
AAGCGAGAACCAATGGATGAAAACTTTGACGATTTAGATGTTTCTTCTGGGAGTGGAGGG
AAAAGACCCACAACAAAGGGACATTTGGAAGCCCCCAACAAATCTTGGTGTTCAGGGATC
ACATAATCCTTGTTTTTATCCAGAAATAGTTCAGACTGATATAGAACCTGGAGATTTAGA
AGACATGAGAAACTTCTGTGTTTTACAAGGTCCATTTTTCTTCAAAGGAGAGAGAACCTA
CTTCTCCAGCATAATGGGCAACAGCGAAATCTGTTCGAGAGAGTTTTGGTTTGATGAACC
GCTTGTGTGTCTTAAAAGTCTGATACAGCTTATTTGCAAATGTTTCGTGGGTTGACTTTG
GAAACATGCTGAAAAATTACACCTCGTTAGTTCAAATCAAGAACATAATCACCTCATGCT
TCATATGGAACTAGTTTGATCTTTAACTTTACCAAGCTTCTTCGAGGAGAGCAACAATTC
CCCCAGGCTTCTGCAAATGGAAAATAAAAGGGGAAATAAAGTGAGATAACAAAATCCACC
CGGTATGTGGAGTTATTTTGTAAACATTGTCCTTTCTCGGTTC
Primers used for PCR AS27 - 8474, show primer details
   
Sequence Name 33-K023624-022-262-B03C-8409
GenBank Accession LN500831 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K023624-022-262-B03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCACAAATTCAATG
TAACTCCAATCTATTGCTTCTTTTGTGTACTCTTCCTGTTCCATCTTGAAAACGTGCTGA
AAAAAAGCCGATTTGAGAATCAGATATGGAATTAATAAACCTTTTTCAACTACACTCACG
AACATACCTGATTAAAATGTTGCTGCAACTTCTCATTAGTGAAATTAATACAGAACTGGT
CAAAACTGCCGCAAAAGGGTATTAACAGAA
   
Sequence Name 34-K023624-022-262-B03C-AS27
GenBank Accession LN501255 [GenBank]
Sequencing primer gene specific primer, AS27: 5'-GTAATGTGCAGGTTGGTAGACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K023624-022-262-B03C-AS27
AATAAGTCGATTGGACAAGATGCCAATTCTAGATCTCTAATTGGAGTTCTGGACATTTAC
GGATTTGAGAGCTTCAAAACTAACAGGTAAATGAACCTTTACCTTGGTTAAGACTAACAG
ACTTGCAGTCTGCCATTCTCTGCTAATACCCTTTTGCTGCAGTTTTGAACAGTTCTGTAT
TAATTTCACTAATGAGAAGTTGCAGCAACATTTTAATCAGGTATGTTCGTGAGTGTAGTT
GAAAAAGGTTTATTAATTCCATATCTGATTCTCAAATCGGCTTTTTTTCAGCACGTTTTC
AAGATGGAACAGGAAGAGTACACAAAAGAAGCAATAGATTGGAGTTACATTGAATTTGTG
GACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTCATCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11