Sequences from confirmation amplicon


Confirmation sequences for line 262E04 and hit At3g02290 are shown.
Line ID 262E04
Locus At3g02290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:460342
Primers used for PCR PP29 - 8474, show primer details
   
Sequence Name 29-K102867-0022-262-E04C-8409
GenBank Accession LN499239 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K102867-0022-262-E04C-8409
ATTTACAATTGAATATATCCTGATTGCTTTGACGGGAGTTTTACCGCACTTCGTTCTAAG
AATGCAGAAAAAAAAAGTGGTTACCTTTCCGCAGACTGGACAGTTTTCACTTCTCTCCAT
CCACTCATAAATGCAACTAAGGTGGAAATGGTGTGAACATTTTGTCACAATCTTTGGGTT
CTCTGATGTATATTCTGCAAAAAACCAACAAGATATGAATACTGAAGGAACAAAAAGGAG
ACTGTTGAATGGGATTATTATTACCTTCAAGACAAGTTGGGCAGACATCTTCGTCTTCAG
ACAATATATACATGTTTTCAGAGTCGGCCATTATTGACTTGGACCTTGATCTCAAAATCC
TGAGACTGGATTTACTAGAGTACTCTTCTTTGGAATCTTTATCAGAGAGGGTGGACTTAT
TATTAGCCCATTTGCACCCTCCTACCCCGAAAGATTCAGAATCTGCATCGCTATCGCTTC
TTAAAGGTTCAACTTCCTCATGAG
   
Sequence Name 29-K102868-0022-262-E04C-PP29
GenBank Accession LN499240 [GenBank]
Sequencing primer gene specific primer, PP29: 5'-TTCTCATGAGGAAGTTGAACCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K102868-0022-262-E04C-PP29
GGCTAATAATAAGTCCACCCCCTATGATAAAGATTCCAAAGAAGAGTACTCTAGTAAATC
CAGTCTCAGGATTTTGAGATCAAGGTCCAAGTCAATAATGGCCGACTCTGAAAACATGTA
TATATTGTCTGAAGACGAAGATGTCTGCCCAACTTGTCTTGAAGGTAATAATAATCCCAT
TCAACAGTCTCCTTTTTGTTCCTTCAGTATTCATATCTTGTTGGTTTTTTGCAGAATATA
CATCAGAGAACCCAAAGATTGTGACAAAATGTTCACACCATTTCCACCTTAGTTGCATTT
ATGAGTGGATGGAGAGAAGTGAAAACTGTCCAGTTTGCGGAAAGGTAACCACTTTTTTTT
TCTGCATTCTCACATCAAAATGCGTATTAATCTCCCGTCAAAGCATTCAGGATATATTCA
ATTGTAATTG

Last Updated on 06.12.2018 16:11