Sequences from confirmation amplicon


Confirmation sequences for line 263C02 and hit At1g06170 are shown.
Line ID 263C02
Locus At1g06170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1886128
Primers used for PCR PH67 - 8474, show primer details
   
Sequence Name 19-K017511-022-263-C02C-PH67
GenBank Accession LN494552 [GenBank]
Sequencing primer gene specific primer, PH67: 5'-GATGTAATCTATCGCTTCTCCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K017511-022-263-C02C-PH67
ATGCTCTATCATTCTAGAGATATAAGAAAAAAGGGTTTAATTAGTTTCTGAAGATTTCTT
ACTTGAAGAAGAAGTTAGTATTTAAGTAAGTGTAAACCTTGGTGGGATTTGGAATTAGAT
TCTTTAAGTCACCAAACCGGTCTTTGAAGTGAACTCTTCTCTCGCGTTCTGTAGGGAAAA
TTTTACGCTTTTTCGATCCTCTGCCTTTACGGTTAAGACCATCGTCGAGGTTGTTGTTTT
CTTCATCATACATCAAGTTCACTCCTTGGTTAGTCTCAGTTTGATCTTGATCCACTCCCA
TGAAACTCCCATTAGGAGGCTGCAGAGGAAAATTCAGATGGAAGAGCGGGTCATAGGCGG
CGACAGAGGAAGATGTTGTCATGATAATGTCTTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 23-K017511-022-263-C02C-8409
GenBank Accession LN496354 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017511-022-263-C02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGACATTATCATGACAACATCT
TCCTCTGTCGCCGCCTATGACCCGCTCTTCCATCTGAATTTTCCTCTGCAGCCTCCTAAT
GGGAGTTTCATGGGAGTGGATCAAGATCAAACTGAGACTAACCAAGGAGTGAACTTGATG
TATGATGAAGAAAACAACAACCTCGACGATGGTCTTAACCGTAAAGGCAGAGGATCGAAA
AAGCGTAAAATTTTCCCTACAGAACGCGAGAGAAGAGTTCACTTCAAAGACCGGTTTGGT
GACTTAAAGAATCTAATTCCAAATCCCACCAAGGTTTACACTTACTTAAATACTAACTTC
TTCTTCAAGTAAGAAATCTTCAGAAACTAATTAAACCCTTTTTTCTTATATCTCTAGAAT
GATAGAGCATCAATCGTCGGAGAAGCGA

Last Updated on 06.12.2018 16:11