Sequences from confirmation amplicon


Confirmation sequences for line 263C05 and hit At2g35160 are shown.
Line ID 263C05
Locus At2g35160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14823701
Primers used for PCR HM50 - 8474, show primer details
   
Sequence Name 18-K032600-022-263-C05C-HM50
GenBank Accession LN494197 [GenBank]
Sequencing primer gene specific primer, HM50: 5'-CTGCATGTCTTCTTTTGTTGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K032600-022-263-C05C-HM50
TCATCATAACATGCATTGCCAAGATATTCAATTTAATTGATTGCAATCTTTTCTAATCAA
TACGCGTTCTTGTAACTACTAGGAATCTAGGATGGTGTTTTTTAATTAATATGCTTTGTT
GTAACTTACTAGGATGGTGTTTTTAGTTCATATTCTTGTATTACATTGAGTAGGCGTTGG
GCATCATGGTACATTCAGAGTCATCAATACTTAGTTCCTTGCGCGGTGGTGATGGCGGTG
GTATTCCTTGTTCTAAGGATGAACTAGCAATAAATGGTAGTTACACCGATCCTATGGGGC
GGAGGAAGTCCAAGCGGTTTAAGGTTGCTGCGGAAAGTGAGTTTTCATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTC
   
Sequence Name 18-K032600-022-263-C05C-8409
GenBank Accession LN494196 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K032600-022-263-C05C-8409
ACCTTAACCGCTTGGACTTTCTCCGCCCCATAGGATCGGTGTAACTACCATTTATTGCTA
GTTCATCCTTAGAACAAGGAATACCACCGCCATCACCACCGCGCAAGGAACTAAGTATTG
ATGACTCTGAATCGTACCATGATGCCCAACGCCTACTCAATGTAATACAAGAATATGAAC
TAAAAACACCATCCTAGTAAGTTACAACAAAGCATATTAATTAAAAAACACCATCCTAGA
TTCCTAGTAGTTACAAGAACGCGTATTGATTAGAAAAGATTGCAATCAATTAAATTGAAT
ATCTTTGGCAATGCATGTTATGATGATCTAAGTTCATCATCACAATTAACCAACAAAA

Last Updated on 06.12.2018 16:11