Sequences from confirmation amplicon


Confirmation sequences for line 263D05 and hit At5g62050 are shown.
Line ID 263D05
Locus At5g62050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24924764
Primers used for PCR PH52 - 8474, show primer details
   
Sequence Name 11-K017511-022-263-D05C-PH52
GenBank Accession LN490899 [GenBank]
Sequencing primer gene specific primer, PH52: 5'-GGAAGTCATCCTCACTTTTGCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K017511-022-263-D05C-PH52
ATAGCTTTTGTACTGACTGCATGTCCACTCAGCTTTAGCTCAGCATGTTAATTTTGATTT
TCTCCATATTCTTTCCTACTATTTTTGTTCAGGGAATGGACTCGGTTACGATGGCAGAAG
GTCAAAAAAAGATGAAGAATTTGTTTAAAGAGTAAGCTGCTTTTAAATTTAAACATTTTC
TGACCAGAAAAATCAAATTTAAAGATCTCTTTGGAGTTTAAACTTCTTTTGGTCGTAAAA
ACTAGAATTCTATACTAGTTTTAATATAAGTTTTCCCTCGTGGATCATAGTTGGGTAGGG
AGTGCGCTATATGTAGCTTGTCTGTTCTACTGGTTATTGTATTGGTGCAGTCTCCTGACA
TTTTGAATATATTGGTAGATATGTCCTAATAACCATATACTGTTTGTTTATAATTTACAG
ATATGGTGTCACTCCATTCACCCCAATGAAAGGGATGTTTATTCAGGGACCGCTGTTCAT
CTGCTTTTTCCTTGCTGTAAGACGTTTTAATCTTAAGATGTTTTTTGGGCTATGTTGTTT
GTTATATCATTAGGCTTTTTGTCTCAAATGAGTTATTTTTTCCCCAGATTCGAAATATGG
CAGAGAAGGTACCTTCATTCCAAACAGGAGGTGCATTATGGTTTACCGATCTAACAACTC
CCGACAGCTTATACATCTTGCCGGTTATAAATGGATCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCCATTTTTTTTCAATTCAAAAA
   
Sequence Name 15-K017511-022-263-D05C-8409
GenBank Accession LN492749 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017511-022-263-D05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGATCCATTTATAACCGGCAAGATGTATA
AGCTGTCGGGAGTTGTTAGATCGGTAAACCATAATGCACCTCCTGTTTGGAATGAAGGTA
CCTTCTCTGCCATATTTCGAATCTGGGGAAAAAATAACTCATTTGAGACAAAAAGCCTAA
TGATATAACAAACAACATAGCCCAAAAAACATCTTAAGATTAAAACGTCTTACAGCAAGG
AAAAAGCAGATGAACAGCGGTCCCTGAATAAACATCCCTTTCATTGGGGTGAATGGAGTG
ACACCATATCTGTAAATTATAAACAAACAGTATATGGTTATTAGGACATATCTACCAATA
TATTCAAAATGTCAGGAGACTGCACCAATACAATAACCAGTAGAACAGACAAGCTACATA
TAGCGCACTCCCTACCCAACTATGATCCACGAGGGAAAACTTATATTAAAACTAGTATAG
AATTCTAGTTTTTACGACCAAAAGAAGTTTAAACTCCAAAGAGATCTTTAAATTTGATTT
TTCTGGTCAGAAAATGTTTAAATTTAAAAGCAGCTTACTCTTTAAACAAATTCTTCATCT
TTTTTTGACCTTCTGCCATCGTAACCGAGTCCATTCCCTGAACAAAAATAGTAGGAAAGA
ATATGGAGAAAATCAAAATTAACATGCTGAGCTAAAGCTGAGTGGACATGCAGTCAGTAC
AAAAGCT

Last Updated on 06.12.2018 16:11