Sequences from confirmation amplicon


Confirmation sequences for line 264A03 and hit At4g11850 are shown.
Line ID 264A03
Locus At4g11850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7130239
Primers used for PCR 37ys - 8474, show primer details
   
Sequence Name 31-K122428-0022-264-A03C-37ys
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 37ys: 5'-TGTGGCTGATACCCTCCCATG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K122428-0022-264-A03C-37ys
ATCTCTGGTTGATATTCGCGGAACCATTAAGACAAACTCATCATCCACTACCATACCTTT
GGAATGAACATATATCATGAATCTTCGACTCTTCAAAGCTTGTACCTAGATTAGTTTGTT
GTTTCAGTGAGTTAACGAGGCCATTATGGTGAAACTCAAGATTTTAAAAAGAAGAAATTT
ATATATGTATACCTGTGCAGCATTTGCATTTGCGTTTGGCTGAGGTGGCTTCCGAGGACT
ATTATATACGCTAACTGTTCCAACAGGAACTTCTCTGGTTCCAAGACAGAAAAAGTTCAA
AAAGTCTTGTGGCTCAAACTGGCTATCAAGACCAACTTCCACAAGTGCCTTGTAGATGGT
TTGATACATCATTGCTGATCCATCATACTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K122427-0022-264-A03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K122427-0022-264-A03C-8409
TCCTGAGTATGATGGATCAGCAATGATGTATCAAACCATCTACAAGGCACTTGTGGAAGT
TGGTCTTGATAGCCAGTTTGAGCCACAAGACTTTTTGAACTTTTTCTGTCTTGGAACCAG
AGAAGTTCCTGTTGGAACAGTTAGCGTATATAATAGTCCTCGGAAGCCACCTCAGCCAAA
CGCAAATGCAAATGCTGCACAGGTATACATATATAAATTTCTTCTTTTTAAAATCTTGAG
TTTCACCATAATGGCCTCGTTAACTCACTGAAACAACAAACTAATCTAGGTACAAGCTTT
GAAGAGTCGAAGATTCATGATATATGTTCATTCCAAAGGTATGGTAGTGGATGATGAGTT
TGTCTTAATTGGTTCCGCGAATATCAACCAGAGATCCTTGGAAGGAACTAGAGATACTGA
AATCGCCATGGGAGGGTATCAGCCAC

Last Updated on 06.12.2018 16:11