Sequences from confirmation amplicon


Confirmation sequences for line 264B04 and hit At2g31660 are shown.
Line ID 264B04
Locus At2g31660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13464378
Primers used for PCR CK31 - 8474, show primer details
   
Sequence Name 22-K017884-022-264-B04C-CK31
GenBank Accession LN495906 [GenBank]
Sequencing primer gene specific primer, CK31: 5'-AGGCATCAGAAACTGCATAACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K017884-022-264-B04C-CK31
ATTGATCATTAGAAATAGTAAACACCACTGAAATCTGGCTACAGTGAATTAAAGCAAATA
ACGATTTGTTCAAAAAGAGTGGGAATCTTTGAACTATCTTCAGTAAAACCAGAAAGTGAA
CTTATAAGGAAACTCTATACATGAACTCAGCTCAAACTTTAAGATACTACTAGCAGAATA
AGAACAATCAAACTCGCGACCAAGCAACCGTGTAAATACATCGAAAGAGAAATTGAAGTG
GAAACTGAGAAACCTGATTGAGTTGCTGCTCAGAAACTTTACGCTCATCGGGAATAAGG
   
Sequence Name 23-K017884-022-264-B04C-8409
GenBank Accession LN496357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017884-022-264-B04C-8409
TAGATTTCTTCTTCTTCTTCGTCATGCTCTTCTGAGATTCTCCCCTCCTCTAATTCAGCT
CTCTCTCTTGGTTGGGAATTTCAAATCGAATATCAACCACTGCCTCTGATCGTCATCAAG
GTTTACTCTGTTTAGTGTCCGGATTCTCAAACTCCACGGAGAATGGATCTGCATAGCCTC
GCTTTGATCCTCCGAACCGCCGCTCTTAGCCCTATTCCCGATGAGCGTAAAGTTTCTGAG
CAGCAACTCAATCAGGTTTCTCAGTTTCCACTTCAATTTCTCTTTCGATGTATTTACACG
GTTGCTTGGTCGCGAGTTTGATTGTTCTTATTCTGCTAGTAGTATCTTAAAGTTTGAGCT
GAGTTCATGTATAGAGTTTCCTTATAAGTTCACTTTCTGGTTTTACTGAAGATAGTTCAA
AGATTCCCACTCTTTTTGAACAAATCGTTATTTGCTTTAATTCACTGTAGCCAGATTTCA
GTGGTGTTTACTATTTCTAATGATCAATTAAAGTTATGCAG

Last Updated on 06.12.2018 16:11