Sequences from confirmation amplicon


Confirmation sequences for line 265C09 and hit At5g37600 are shown.
Line ID 265C09
Locus At5g37600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14934435
Primers used for PCR NK60 - 8474, show primer details
   
Sequence Name 43-K103393-0022-265-C09C-NK60
GenBank Accession LN504804 [GenBank]
Sequencing primer gene specific primer, NK60: 5'-GCCATGCTTTATTACATCCATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K103393-0022-265-C09C-NK60
GCGAGCGTACACTCCCGCGGGTGAACCATCCCGACTAACAAAAGACACGCTGCGGCTAAG
GTCTTTAGCAACCCTGATGTTGCAGCTGAAGTGCCATGGTATGGTATTGAGCAAGAATAC
ACTTTACTCCAGAAAGATGTGAAGTGGCCTGTTGGTTGGCCTATTGGTGGTTATCCCGGC
CCTCAGGTGAAGAAGATGGATATATAATCGGTTTTGTATCTTTTCCGTTGTCGGTTATTC
TAAAATTTTAGGCGAAAATGTATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCACGATTATA
   
Sequence Name 33-K103394-0022-265-C09C-8409
GenBank Accession LN500959 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103394-0022-265-C09C-8409
CATTTACATTGAATATATCCTGCAATACATTTTCGCCTAAAATTTTAGAATAACCGACAA
CGGAAAAGATACAAAACCGATTATATATCCATCTTCTTCACCTGAGGGCCGGGATAACCA
CCAATAGGCCAACCAACAGGCCACTTCACATCTTTCTGGAGTAAAGTGTATTCTTGCTCA
ATACCATACCATGGCACTTCAGCTGCAACATCAGGGTTGCTAAAGACCTTAGCCGCAGCG
TGTCTTTTGTTAGTCGGGATTGGTTCACCCGCGGGAGTGTACGCATCGCANATGACCTGA
TCAAAACCATTAAGTTAAGATGGATGTAATAAAGCATGG

Last Updated on 06.12.2018 16:11