Sequences from confirmation amplicon


Confirmation sequences for line 265D09 and hit At5g19580 are shown.
Line ID 265D09
Locus At5g19580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6608537
Primers used for PCR 14kh - 8474, show primer details
   
Sequence Name 34-K113531-0022-265-D09C-14kh
GenBank Accession LN501486 [GenBank]
Sequencing primer gene specific primer, 14kh: 5'-ACGTTTAGCTCCGTTGACTAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113531-0022-265-D09C-14kh
ATTCGTGGGGTTGGCATCATCTCCGTCTTCCATCGTGGCTTTGCGCTGTTGATCCTTATC
CTGGCGCAGTCTTGTAGTGCTGGTTCGTATATTTTTTTACCAGCCTTGTAATACGCGTCT
TGTTTGGAACCACCACAGACGAGAACCTCGGCTGGGATGACCTTGGGGTTTTTCACGTAA
AGTTGGATGGGGAGGAGTGCGGAAGAACCTGATCCCGGGTAGTTACGGGCACCACCTGGG
AGCTGAGGGAACTCCTTGATGACTTGGTTTGTTTTGGGGCTTAGAAGGATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 09-K113530-0022-265-D09C-8409
GenBank Accession LN490260 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113530-0022-265-D09C-8409
CCTTCTAGCCCCAAAACAAACCAAGTCATCAAGGAGTTCCCTCAGCTCCCAGGTGGTGCC
CGTAACTACCCGGGATCAGGTTCTTCCGCACTCCTCCCCATCCAACTTTACGTGAAAAAC
CCCAAGGTCATCCCAGCCGAGGTTCTCGTCTGTGGTGGTTCCAAACAAGACGCGTATTAC
AAGGCTGGTAAAAAAATATACGAACCAGCACTACAAGACTGCGCCAGGATAAGGATCAAC
AGCGCAAAGCCACGATGGAAGACGGAGATGATGCCAACCCCACGAATCATGAGTGATACT
GTCATCCTCCCTAACGGAGACATCCTTCTAGTCAACGGAACTAAACGTA

Last Updated on 06.12.2018 16:11