Sequences from confirmation amplicon


Confirmation sequences for line 266B08 and hit At4g14710 are shown.
Line ID 266B08
Locus At4g14710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8425344
Primers used for PCR 27sm - 8474, show primer details
   
Sequence Name 44-K116453-0022-266-B08C-27sm
GenBank Accession LN505304 [GenBank]
Sequencing primer gene specific primer, 27sm: 5'-GTCTTTTCTTGGATGAACTTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116453-0022-266-B08C-27sm
GGAACAGACATTGTACCTTGCAGGGAGGTGGTCGTGTGGGCGATTGTATGGTGTCCAGAC
CGGTTCACCCACGAAAAGCCGCATTGCCTGCTCAACAAGATATTTCAATTTTCTAATCAA
CCAAATATATATATGATGAATTAACCACTCTGAAGAAGCTAACATTGCACCTTGATATAG
TTGTCTGAGTCTACGGTGAAGCGATGATATATTCCAGCAGGTAAGACTATCATACCTCCC
TTCTTTACCCATACTCTAATCCAAGCTTCATTGCGATCCCTCACATCCGGGACAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAAGGTTATTATAAT
   
Sequence Name 11-K116452-0022-266-B08C-8409
GenBank Accession LN491280 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116452-0022-266-B08C-8409
ATTTACATTGATATATCCTGTCCCGGATGTGAGGGATCGCAATGAAGCTTGGATTAGAGT
ATGGGTAAAGAAGGGAGGTATGATAGTCTTACCTGCTGGAATATATCATCGCTTCACCGT
AGACTCAGACAACTATATCAAGGTGCAATGTTAGCTTCTTCAGAGTGGTTAATTCATCAT
ATATATATTTGGTTGATTAGAAAATTGAAATATCTTGTTGAGCAGGCAATGCGGCTTTTC
GTGGGTGAACCGGTCTGGACACCATACAATCGCCCACACGACCACCTCCCTGCAAGGTAC
AATGTTCTGTTTTCCCCTTTTGTCTCATCAAAAACTTACTCTGCAAAAGTTCATCCAAGG
AAAAGACA

Last Updated on 06.12.2018 16:11