Sequences from confirmation amplicon


Confirmation sequences for line 266D03 and hit At2g44540 are shown.
Line ID 266D03
Locus At2g44540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18387238
Primers used for PCR KR52 - 8474, show primer details
   
Sequence Name 60-K100821-0022-266-D03C-8409
GenBank Accession LN510195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K100821-0022-266-D03C-8409
CATTTACAATTGAATACTCATTGCTCATCTGGTCGTTAGTAGCACGGTGGAGCCAAGCTG
CAGCCCACAACAACTCATCTTCGTATCCACTACTCGCGTAGAAGCCACCCGCGTTAGTAA
TGGAGTTTTGGTAAAGGCCAGGGTGAGCCTTAGCGAATGCAAATAAATCTTTTGCATGAC
CAATGAGTATATTGGCATAGGCTTTATCAAATGGTGCGAAGGCGATAGACGCTGCCGCCA
TTGCGGCAGCTGTCTCACCTGCTAGGTCAGAACCTGGATGTTGAGCATCGATGCGGTATG
AAGTACGCGGAGTGGTCATATCCTCAGGTCTCATCCAGCATTTGTGGTCCGAGTTTCCCT
CACCCACTTGTCCGTACAGGACATTAGGCTGAGGATGGGCTTTAATGAGATAATCGGTGG
CCCACTTGAGAGCCCTGAGCGAGTTTCCTTGCTCGTGGTGGGCCCTAAGCTCTGATCCCA
TCTCTATGTTGCTCCAAGCTAGCATCGTCGTAGTGAACGCTAAAGGAAATCCAAACTTCA
ATTGGTCTCCG
   
Sequence Name 60-K100833-0022-266-D03C-KR52
GenBank Accession LN510197 [GenBank]
Sequencing primer gene specific primer, KR52: 5'-CGGAGACAATATGAAGTTTGGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K100833-0022-266-D03C-KR52
ACATAGAGATGGGATCAGAGCTTAGGGCCCACCACGAGCAAGGAAACTCGCTCAGGGCTC
TCAAGTGGGCCACCGATTATCTCATTAAAGCCCATCCTCAGCCTAATGTCCTGTACGGAC
AAGTGGGTGAGGGAAACTCGGACCACAAATGCTGGATGAGACCTGAGGATATGACCACTC
CGCGTACTTCATACCGCATCGATGCTCAACATCCAGGTTCTGACCTAGCAGGTGAGACAG
CTGCCGCAATGGCGGCAGCGTCTATCGCCTTCGCACCATTTGATAAAGCCTATGCCAATA
TACTCATTGGTCATGCAAAAGATTTATTTGCATTCGCTAAGGCTCACCCTGGCCTTTACC
AAAACTCCATTACTAACGCGGGTGGCTTCTACGCGAGTAGTGGATACGAAGATGAGTTGT
TGTGGGCTGCAGCTTGGCTCCACCGTGCTACTAACGACCAGATGAGCAATGAGTATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11